NICOTINIC ACETYLCHOLINE-RECEPTORS ON THE SURFACE OF THE BLOOD FLUKE SCHISTOSOMA
NICOTINIC ACETYLCHOLINE-RECEPTORS ON THE SURFACE OF THE BLOOD FLUKE SCHISTOSOMA
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DOI：
10.1016/0166-6851(94)00039-p
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发表时间：
1995-04-01
影响因子：
1.5
通讯作者：
AGNEW, A
中科院分区：
文献类型：
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作者：
CAMACHO, M;ALSFORD, S;AGNEW, A
血吸虫的体表有乙酰胆碱酯酶(AChE)。作为了解被膜AChE功能的第一步，我们使用了特异性配体结合试验来鉴定血吸虫表面的烟碱型乙酰胆碱受体(NAChR)。在异硫氰酸荧光素-α-银环蛇毒素结合的迁徙阶段，不能检测到AchR，但随着寄生虫成熟到产卵成虫，随着有性配对和成虫的成熟，AchR的特异性标记量增加。AChE和nAChR均集中在成年男性背侧。这些结果表明，AChE和AChR的作用与该膜的转运功能有关。
Blood dwelling stages of schistosomes have acetylcholinesterase (AChE) on their teguments. As an initial step towards understanding the function of tegumental AChE, we have used specific ligand-binding assays to identify nicotinic acetylcholine receptors (nAChR) on the schistosome surface. AChR could not be detected on migratory stages using fluoroscein isothiocyanate-alpha-bungarotoxin binding but the amount of specific labelling increased on sexual pairing and as the parasites matured into egg-producing adults. Both AChE and nAChR were concentrated on the dorsal surface of the adult male. These results indicate a role for AChE and AChR associated with the transporting function of this membrane.