ISOLATION AND AMINO-ACID-COMPOSITION OF 2 SOMATOSTATIN-LIKE PEPTIDES FROM OVINE HYPOTHALAMUS - SOMATOSTATIN-28 AND SOMATOSTATIN-25
ISOLATION AND AMINO-ACID-COMPOSITION OF 2 SOMATOSTATIN-LIKE PEPTIDES FROM OVINE HYPOTHALAMUS - SOMATOSTATIN-28 AND SOMATOSTATIN-25
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DOI：
10.1016/0006-291x(80)91415-1
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发表时间：
1980-01-01
影响因子：
3.1
通讯作者：
GUILLEMIN, R
中科院分区：
文献类型：
--
作者：
BOHLEN, P;BRAZEAU, P;GUILLEMIN, R
通过亲和层析、凝胶过滤、阳离子交换层析和反相高效液相层析，从绵羊下丘脑提取物中纯化出两种具有类生长抑素免疫活性和体外生物活性的肽。两种肽均含有生长抑素的所有氨基酸；因此，它们可能在结构上与十四肽生长抑素相关。其中一种肽含有 28 个氨基酸，其组成与生长抑素 28 相同，生长抑素是最近从猪肠中分离出来的 N 末端延伸的生长抑素，并由 Pradayrol 等人表征。 （1980）。第二个肽，生长抑素-25，少了 3 个氨基酸残基，但其他方面与生长抑素-28 相同。根据体外生物测定和氨基酸分析定量，这两种肽似乎比生长抑素更有效。
Two peptides having somatostatin-like immunoactivity and in vitro bioactivity were purified to homogeneity from ovine hypothalamic extracts by affinity chromatography, gel filtration, cation exchange chromatography and reverse-phase high-performance liquid chromatography. Both peptides contain all the amino acids of somatostatin; thus it is likely that they are structurally related to the tetradecapeptide somatostatin. One of the peptides contains 28 amino acids with a composition identical to that of somatostatin-28, the N-terminally extended somatostatin recently isolated from porcine intestine and characterized by Pradayrol, et al. (1980). The 2nd peptide, somatostatin-25, has 3 amino acid residues less but otherwise the same composition as somatostatin-28. Based on an in vitro bioassay and quantitation by amino acid analysis both peptides appear to be more potent than somatostatin.