Bacterial overexpression of putative yeast mitochondrial transport proteins.
Bacterial overexpression of putative yeast mitochondrial transport proteins.
复制标题
假定的酵母线粒体转运蛋白的细菌过度表达。
DOI：
10.1023/a:1022426900735
复制
发表时间：
1997
影响因子：
3
通讯作者：
Kaplan,RS
中科院分区：
文献类型：
--
作者：
Mayor,JA;Kakhniashvili,D;Gremse,DA;Campbell,C;Krämer,R;Schroers,A;Kaplan,RS
Thirty-two genes have been identified within the genome of the yeastSaccharomyces cerevisiaewhich putatively encode mitochondrial transport proteins. We have attempted to overexpress a subset of these genes, namely those which encode mitochondrial transporters of unknown function, and have succeeded in overexpressing 19 of these genes. The overexpressed proteins were then isolated and tested for five well-characterized reconstituted transport activities (i.e., the transport of citrate, dicarboxylates, pyruvate, camitine, and aspartate). Utilizing this approach, we have clearly identified the yeast mitochondrial dicarboxylate transport protein, as well as two additional lower-magnitude transport functions (i.e., tricarboxylate and dicarboxylate transport activities). The implications of these results and the considerations relevant to this approach are discussed.
