Prevalence of virulence genes among bulgarian nosocomial and cystic fibrosis isolates of pseudomonas aeruginosa.
Prevalence of virulence genes among bulgarian nosocomial and cystic fibrosis isolates of pseudomonas aeruginosa.
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DOI：
10.1590/s1517-83822010000300008
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发表时间：
2010-07
期刊：
影响因子：
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通讯作者：
Markova B
中科院分区：
文献类型：
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作者：
Mitov I;Strateva T;Markova B
本研究的目的是评估囊性纤维化 (CF) 患者 (n=42) 和非 CF 住院患者 (n=160) 的 202 株铜绿假单胞菌分离株中某些毒力基因的流行率，并根据患者群体、感染部位和抗菌药物耐药性分析这些值。所有研究菌株中的频率如下：algD（编码 GDP-甘露糖 6-脱氢酶 AlgD）– 91.1%，pilB（IV 型菌毛生物发生蛋白 PilB）– 23.8%，nan1（神经氨酸酶）– 21.3%，lasB（弹性蛋白酶 LasB）– 100%，plcH（溶血磷脂酶 C 前体） – 91.6%，exoS（外酶 S） – 62.4%，exoU（外酶 U） – 30.2%。 CF分离株中nan1的患病率（38.1%）显着高于非CF分离株（16.9%）（P<0.01）。 nan1 阳性 CF 菌株是从 16 名肺部反复发作的患者中培养出来的。本研究显示，含有大量（≥5）毒力基因的多重耐药（MDR）院内铜绿假单胞菌菌株部分（38.1%）与非MDR分离株相应部分（17.6%）之间存在统计学显着差异（P<0.01）。此外，pilB、exoU 和 nan1 在 MDR 菌株中的传播率高于非 MDR 菌株（分别为 39.1% vs. 13.2%；40.2% vs. 17.7% 和 26.1% vs. 4.4%）。总之，nan1 在 CF 分离株中的传播是中等的，并且与肺部病情恶化的患者比例较低相关。该基因的分子遗传学检测可用作 CF 肺部疾病演变的间接测量。同时测定毒力因子和抗菌素耐药性是检查铜绿假单胞菌引起的感染的微生物学方面的当代方法。
The aim of this study was to evaluate the prevalence of some virulence genes among 202 Pseudomonas aeruginosa isolates from cystic fibrosis (CF) patients (n=42) and non-CF in-patients (n=160) and to analyze the values according to the patient groups, infection localization and antimicrobial resistance. The following frequencies in all studied strains were established: algD (encoding GDP-mannose 6-dehydrogenase AlgD) – 91.1%, pilB (type IV fimbrial biogenesis protein PilB) – 23.8%, nan1 (neuraminidase) – 21.3%, lasB (elastase LasB) – 100%, plcH (haemolytic phospholipase C precursor) – 91.6%, exoS (exoenzyme S) – 62.4%, and exoU (exoenzyme U) – 30.2%. The prevalence of nan1 was significantly higher (P<0.01) in CF isolates (38.1%) than that in non-CF isolates (16.9%). The nan1–positive CF strains were cultured from 16 patients with recurrent lung exacerbations. This study revealed a statistically significant difference (P<0.01) between the portion of multidrug-resistant (MDR) nosocomial P. aeruginosa strains containing a large number (≥5) of virulence genes (38.1%) and the respective part of non-MDR isolates (17.6%). Moreover, pilB, exoU and nan1 manifested a higher spread (P<0.001) among MDR than in non-MDR strains (respectively, 39.1% vs. 13.2%; 40.2% vs. 17.7% and 26.1% vs. 4.4%). In conclusion, the dissemination of nan1 in CF isolates was moderate and correlated with the lower proportion of patients with lung exacerbations. The molecular-genetic detection of this gene may be used as an indirect measure of CF pulmonary disease evolution. Simultaneous determination of virulence factors and antimicrobial resistance is the contemporary approach for examination of the microbiological aspects of infections caused by P. aeruginosa.