Alternate wiring of a KNOXI genetic network underlies differences in leaf development of A. thaliana and C. hirsuta.
Alternate wiring of a KNOXI genetic network underlies differences in leaf development of A. thaliana and C. hirsuta.
复制标题
DOI：
10.1101/gad.269050.115
复制
发表时间：
2015-11-15
影响因子：
10.5
通讯作者：
Tsiantis M
中科院分区：
文献类型：
--
作者：
Rast-Somssich MI;Broholm S;Jenkins H;Canales C;Vlad D;Kwantes M;Bilsborough G;Dello Ioio R;Ewing RM;Laufs P;Huijser P;Ohno C;Heisler MG;Hay A;Tsiantis M
在这项研究中，Rast-Somssich等人研究了C。hirsuta及其近缘种A. thaliana，它有简单的叶子。通过将单个基因从一个物种转移到另一个物种的内源性调控元件下，作者表明，叶的形式可以在受体物种中进行修改，扩展了我们对复杂真核生物中旁系同源基因如何调控的知识。生物学中两个相互关联的问题是理解形态进化的调控逻辑和可预测性。在这里，我们研究了这些问题，通过比较拟南芥，它有简单的叶子，和它的亲戚，碎米荠，它有解剖的叶子，包括小叶。通过两个物种之间的基因转移，我们提供的证据表明，多效性的SHOOTMERISTEMLESS（STM）和BREVIPEDICELLUS（BP）同源框基因和他们的能力，修改叶的形式之间的反比关系。我们进一步表明，BP的顺式调节分歧的结果在两个替代配置的遗传网络控制叶发育。In C. hirsuta中，ChBP被microRNA 164 A（MIR 164 A）/ChCUP形子叶（ChCUC）模块和ChASYMMETRIC LEAVES 1（ChAS 1）抑制，从而在MIR 164 A/CUC和AS 1之间产生在A中不发生的串扰。thaliana.这些不同的遗传结构导致网络组件和生长调节在每个物种中的不同的相互作用。我们认为，某些具有低多效性的调控基因易于整合或脱离影响器官几何形状的保守遗传网络，从而迅速改变其特性，并有助于形态学的分歧。
In this study, Rast-Somssich et al. investigated morphological differences between C. hirsuta, which has complex leaves with leaflets, and its relative, A. thaliana, which has simple leaves. By transferring single genes from one species into another under their endogenous regulatory elements, the authors show that leaf form can be modified in the recipient species, extending our knowledge of how paralogous genes are regulated in a complex eukaryote. Two interrelated problems in biology are understanding the regulatory logic and predictability of morphological evolution. Here, we studied these problems by comparing Arabidopsis thaliana, which has simple leaves, and its relative, Cardamine hirsuta, which has dissected leaves comprising leaflets. By transferring genes between the two species, we provide evidence for an inverse relationship between the pleiotropy of SHOOTMERISTEMLESS (STM) and BREVIPEDICELLUS (BP) homeobox genes and their ability to modify leaf form. We further show that cis-regulatory divergence of BP results in two alternative configurations of the genetic networks controlling leaf development. In C. hirsuta, ChBP is repressed by the microRNA164A (MIR164A)/ChCUP-SHAPED COTYLEDON (ChCUC) module and ChASYMMETRIC LEAVES1 (ChAS1), thus creating cross-talk between MIR164A/CUC and AS1 that does not occur in A. thaliana. These different genetic architectures lead to divergent interactions of network components and growth regulation in each species. We suggest that certain regulatory genes with low pleiotropy are predisposed to readily integrate into or disengage from conserved genetic networks influencing organ geometry, thus rapidly altering their properties and contributing to morphological divergence.