Transgeneration memory of stress in plants
Transgeneration memory of stress in plants
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DOI：
10.1038/nature05022
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发表时间：
2006-08-31
期刊：
影响因子：
64.8
通讯作者：
Hohn, Barbara
中科院分区：
文献类型：
--
作者：
Molinier, Jean;Ries, Gerhard;Hohn, Barbara
由于其固着的性质，植物不断地暴露于大量的环境压力，它们以一系列的反应作出反应。结果是植物对过度或不足的光照、水、盐和温度等条件的耐受性，以及对病原体的抗性。不仅植物生理学已知在非生物或生物胁迫下发生变化，而且基因组的变化也已被确定（1-5）。然而，没有确定来自原始胁迫植物的连续世代的植物是否遗传了基因组变化的能力。在这里，我们表明，在用短波长辐射（紫外线-C）或鞭毛蛋白（植物防御的激发子（6））处理的拟南芥植物中，转基因报告基因的体细胞同源重组在处理过的群体中增加，并且这些增加的同源重组水平持续存在在随后的未处理的世代中。增强型同源重组的表观遗传性状可以通过母本和父本杂交伴侣传递，并被证明是显性的。在处理的世代之后的世代中超重组状态的增加与处理的植物中转基因等位基因（所考虑的重组底物）的存在无关。我们的结论是，环境因素导致增加基因组的灵活性，即使在连续的，未经处理的世代，并可能增加适应的潜力。
Owing to their sessile nature, plants are constantly exposed to a multitude of environmental stresses to which they react with a battery of responses. The result is plant tolerance to conditions such as excessive or inadequate light, water, salt and temperature, and resistance to pathogens. Not only is plant physiology known to change under abiotic or biotic stress, but changes in the genome have also been identified(1-5). However, it was not determined whether plants from successive generations of the original, stressed plants inherited the capacity for genomic change. Here we show that in Arabidopsis thaliana plants treated with short-wavelength radiation (ultraviolet-C) or flagellin ( an elicitor of plant defences(6)), somatic homologous recombination of a transgenic reporter is increased in the treated population and these increased levels of homologous recombination persist in the subsequent, untreated generations. The epigenetic trait of enhanced homologous recombination could be transmitted through both the maternal and the paternal crossing partner, and proved to be dominant. The increase of the hyper-recombination state in generations subsequent to the treated generation was independent of the presence of the transgenic allele ( the recombination substrate under consideration) in the treated plant. We conclude that environmental factors lead to increased genomic flexibility even in successive, untreated generations, and may increase the potential for adaptation.