RNAi-mediated knockdown of the voltage gated sodium ion channel TcNav causes mortality in Tribolium castaneum.
RNAi-mediated knockdown of the voltage gated sodium ion channel TcNav causes mortality in Tribolium castaneum.
复制标题
RNAi介导的电压门控钠离子通道TCNAV的敲低导致Tribolium Castaneum死亡。
DOI：
10.1038/srep29301
复制
发表时间：
2016-07-14
影响因子：
4.6
通讯作者：
Edwards MG
中科院分区：
文献类型：
--
作者：
Abd El Halim HM;Alshukri BM;Ahmad MS;Nakasu EY;Awwad MH;Salama EM;Gatehouse AM;Edwards MG
电压门控钠离子通道（VGSC）属于最大的离子通道超家族。由于VGSC在生理过程中发挥关键作用，因此它们是有效杀虫剂的主要靶标。RNA干扰（RNAi）被广泛用于分析基因功能，但最近，它已显示出潜在的贡献，选择性地控制农业害虫的新策略。本研究评估了靶向赤拟谷盗钠离子通道麻痹A（TcNav）基因的dsRNA的递送作为控制这种昆虫害虫的可行手段。TcNav dsRNA的递送引起严重的发育停滞，注射dsRNA后幼虫死亡率高达73%。注射的幼虫在30- 60%之间的基因表达中显示出显著（p < 0.05）的敲低。在注射后蛹中的表达也显著降低（p < 0.05），分别导致蛹早期和蛹晚期的30%和42%敲减。口服递送dsRNA导致19 - 51.34%的剂量依赖性死亡率;这伴随着连续喂养3天后基因表达的显著（p < 0.05）敲低。注射或喂食dsRNA的大多数幼虫在化蛹前的最后幼虫阶段死亡。这项工作为基于RNAi的昆虫控制策略提供了证据。
The voltage-gated sodium ion channel (VGSC) belongs to the largest superfamily of ion channels. Since VGSCs play key roles in physiological processes they are major targets for effective insecticides. RNA interference (RNAi) is widely used to analyse gene function, but recently, it has shown potential to contribute to novel strategies for selectively controlling agricultural insect pests. The current study evaluates the delivery of dsRNA targeted to the sodium ion channel paralytic A (TcNav) gene in Tribolium castaneum as a viable means of controlling this insect pest. Delivery of TcNav dsRNA caused severe developmental arrest with larval mortalities up to 73% post injection of dsRNA. Injected larvae showed significant (p < 0.05) knockdown in gene expression between 30–60%. Expression was also significantly (p < 0.05) reduced in pupae following injection causing 30% and 42% knockdown for early and late pupal stages, respectively. Oral delivery of dsRNA caused dose-dependant mortalities of between 19 and 51.34%; this was accompanied by significant (p < 0.05) knockdown in gene expression following 3 days of continuous feeding. The majority of larvae injected with, or fed, dsRNA died during the final larval stage prior to pupation. This work provides evidence of a viable RNAi-based strategy for insect control.