Enhancement of histological detail using metanil yellow as counterstain in periodic acid Schiff's hematoxylin staining of glycol methacrylate tissue sections.
Enhancement of histological detail using metanil yellow as counterstain in periodic acid Schiff's hematoxylin staining of glycol methacrylate tissue sections.
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DOI：
10.3109/10520299109109964
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Iliana Quintero-Hunter;Harry Grier;Marilou Muscato
中科院分区：
文献类型：
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作者：
Iliana Quintero-Hunter;Harry Grier;Marilou Muscato
通过用间胺黄稀释溶液复染PAS/铁-苏木素染色切片，改善了包埋在乙二醇甲基丙烯酸酯中的组织切片的组织学细节。复染剂的添加增加了组织切片的对比度，并突出显示PAS阳性实体。染色方案提供了组织形态的清晰定义，区分了细胞类型和其他组织成分，并且不会产生背景染色。
Histological detail in sections from tissues embedded in glycol methacrylate was improved by counterstaining PAS/iron-hematoxylin stained sections with a dilute solution of metanil yellow. The addition of the counterstain increases contrast in tissue sections and highlights PAS-positive entities. The staining protocol provides sharp definition of tissue morphology, differentiates cell types and other tissue components and does not produce background staining.