Iron acquisition from Pseudomonas aeruginosa siderophores by human phagocytes:: an additional mechanism of host defense through iron sequestration?
Iron acquisition from Pseudomonas aeruginosa siderophores by human phagocytes:: an additional mechanism of host defense through iron sequestration?
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DOI：
10.1128/iai.68.3.1271-1275.2000
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发表时间：
2000-03-01
影响因子：
3.1
通讯作者：
Cox, CD
中科院分区：
文献类型：
--
作者：
Britigan, BE;Rasmussen, GT;Cox, CD
铁与铁结合蛋白的螯合是宿主防御的一种策略。一些病原体通过分泌低分子铁络合剂(铁载体)来对抗这种情况，人类吞噬细胞具有从低分子铁络合物中获取铁的高容量机制，宿主吞噬细胞有效地获取和隔离与细菌铁载体结合的铁可以提供第二种机制来限制微生物对铁的获取。在目前的工作中，我们报告了人类中性粒细胞、巨噬细胞和髓系细胞系可以从两个铜绿假单胞菌铁质载体中获得铁。类似于从其他低分子螯合物中获取铁的过程，从铁载体获取铁是不依赖于ATP的，由多价阳离子金属诱导，并且不受体内内吞和胞饮作用抑制剂的影响，在体内，这一过程可以作为另一种宿主防御机制，限制铁对入侵的铁载体产生的微生物的有效性。
Chelation of iron to iron-binding proteins is a strategy of host defense. Some pathogens counter this via the secretion of low-molecular-weight iron-chelating agents (siderophores), Human phagocytes possess a high-capacity mechanism for iron acquisition from low-molecular-weight iron chelates, Efficient acquisition and sequestration of iron bound to bacterial siderophores by host phagocytes could provide a secondary mechanism to limit microbial access to iron. In the present work we report that human neutrophils, macrophages, and myeloid cell lines can acquire iron from the two Pseudomonas aeruginosa siderophores. Analogous to iron acquisition from other low-molecular-weight chelates, iron acquisition from the siderophores is ATP independent, induced by multivalent cationic metals, and unaffected by inhibitors of endocytosis and pinocytosis, In vivo, this process could serve as an additional mechanism of host defense to limit iron availability to invading siderophore-producing microbes.