B-CELL SUB-POPULATIONS IDENTIFIED BY 2-COLOR FLUORESCENCE ANALYSIS
B-CELL SUB-POPULATIONS IDENTIFIED BY 2-COLOR FLUORESCENCE ANALYSIS
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DOI：
10.1038/297589a0
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发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
HERZENBERG, LA
中科院分区：
文献类型：
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作者：
HARDY, RR;HAYAKAWA, K;HERZENBERG, LA
同时快速测量结合在单个细胞上的两种不同的荧光偶联抗体（双色免疫荧光）的数量，为鉴定淋巴细胞亚群提供了一种非常有力的手段1。使用双激光荧光激活细胞分选器（FACS），我们发现两种单克隆抗体，抗igm和抗igd，分别用荧光和“德克萨斯红”（一种新的红色荧光染料）标记，揭示了小鼠脾脏和淋巴结中几个以前未被识别的b细胞亚群。单独测量，这些表面标记物（IgM和IgD）仅显示B细胞在表面免疫球蛋白表达量方面具有广泛的异质性2；然而，同时测量，它们清楚地定义了至少两个b细胞亚群。其中一种主要存在于脾脏并构成淋巴结中绝大多数B细胞的群体，在已知存在B细胞免疫应答缺陷的CBA/N （Xid）小鼠中缺失。
The simultaneous and rapid measurement of the amounts of two different fluorochrome-coupled antibodies bound to single cells (two-colour immunofluorescence) provides a very powerful means for the identification of lymphocyte subpopulations1. Using a dual-laser fluorescence-activated cell sorter (FACS) we show that two monoclonal antibodies, anti-IgM and anti-IgD, labelled respectively with fluorescent and ‘Texas red’ (a new red-fluorescent dye) reveal several previously unrecognized B-cell subpopulations in mouse spleen and lymph nodes. Measured individually, these surface markers (IgM and IgD) show only that B cells are broadly heterogeneous with respect to the amount of surface immunoglobulin expressed2; however, measured simultaneously, they clearly define at least two B-cell subsets. One of these populations, which is predominant in spleen and constitutes the overwhelming majority of B cells in lymph nodes, is missing in CBA/N (Xid) mice known to be deficient with respect to their B-cell immune responses3.