Effects of laeA deletion on Aspergillus flavus conidial development and hydrophobicity may contribute to loss of aflatoxin production
Effects of laeA deletion on Aspergillus flavus conidial development and hydrophobicity may contribute to loss of aflatoxin production
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DOI：
10.1016/j.funbio.2011.12.003
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发表时间：
2012-02-01
期刊：
影响因子：
2.5
通讯作者：
Ingber, Bruce F.
中科院分区：
文献类型：
--
作者：
Chang, Perng-Kuang;Scharfenstein, Leslie L.;Ingber, Bruce F.
构巢曲霉的LaeA是一种假定的甲基转移酶，也是天鹅绒复合物的一个组分;它被认为主要影响次级代谢产物产生所需基因的表达。我们发现，虽然黄曲霉CA 14 laeA缺失突变体显示没有黄曲霉毒素的生产，表达的一些早期基因参与黄曲霉毒素的形成，但不是后来的基因，仍然可以检测到。突变体生长在基本培养基中补充简单的糖和一些复杂的媒体表现出改变分生孢子的发展。在马铃薯葡萄糖琼脂（PDA）培养基上的缺失突变体表现出减少分生孢子链的伸长，分生孢子的生产增加，并降低菌落疏水性相比，亲本菌株。laeA缺失突变体的疏水性丧失和其他发育变化可能影响真菌产生黄曲霉毒素的能力。由Elsevier Ltd代表英国真菌学会出版。
LaeA of Aspergillus nidulans is a putative methyltransferase and a component of the velvet complex; it is thought to mainly affect expression of genes required for the production of secondary metabolites. We found that although Aspergillus flavus CA14 laeA deletion mutants showed no aflatoxin production, expression of some of the early genes involved in aflatoxin formation, but not the later genes, could still be detected. The mutants grown in minimal medium supplemented with simple sugars and on some complex media exhibited altered conidial development. On potato dextrose agar (PDA) medium the deletion mutants showed reduced conidial chain elongation, increased production of conidiophores, and decreased colony hydrophobicity when compared to the parental strain. The loss of hydrophobicity and the other developmental changes in the laeA deletion mutants could affect the ability of the fungus to produce aflatoxins. Published by Elsevier Ltd on behalf of The British Mycological Society.