Identification and Expression Analysis of GRAS Transcription Factors to Elucidate Candidate Genes Related to Stolons, Fruit Ripening and Abiotic Stresses in Woodland Strawberry (Fragaria vesca)
Identification and Expression Analysis of GRAS Transcription Factors to Elucidate Candidate Genes Related to Stolons, Fruit Ripening and Abiotic Stresses in Woodland Strawberry (Fragaria vesca)
复制标题
GRAS 转录因子的鉴定和表达分析，以阐明与林地草莓（Fragaria vesca）匍匐茎、果实成熟和非生物胁迫相关的候选基因
DOI：
10.3390/ijms20184593
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发表时间：
2019-09-02
影响因子：
5.6
通讯作者：
Wu, Han
中科院分区：
文献类型：
--
作者：
Chen, Hong;Li, Huihui;Wu, Han
栽培草莓（Fragaria x ananassa）是蔷薇科的一种重要园艺作物，是一种非跃变型的异源二倍体，果实肉质，主要通过匍匐茎繁殖。林地草莓（Fragaria vesca）是栽培草莓的四个二倍体祖先种之一，是蔷薇科果树、非跃变型果实和匍匐茎研究的模式植物。一个GRAS转录因子已被证明可以调节匍匐茎的形成;林地草莓中的其他GRAS蛋白仍然未知。在本研究中，我们鉴定了林地草莓中的54个FveGRAS蛋白，并将其分为14个亚家族。保守基序分析表明，FveGRAS蛋白的基序组成在每个亚家族内都是保守的，但在亚家族之间差异很大。结果表明，在林地草莓和拟南芥之间共发现56对GRAS蛋白，在林地草莓和水稻之间共发现47对GRAS蛋白，在林地草莓内部共发现92对旁系同源蛋白。FveGRAS基因在各种器官和组织中的表达模式，以及在冷、热和GA（3）处理下的变化，使用转录组学分析来表征。结果表明，34个FveGRAS基因在包括匍匐茎在内的至少4个器官中均有不同程度的表达，只有少数基因表现出器官特异性表达。在果实成熟过程中，16个基因的表达量下降，4个基因的表达量上升，其中FveGRAS 54的表达量上升幅度最大。在冷、热和GA（3）处理下，约一半的FveGRAS基因表现出不同程度的表达增加或减少，表明这些FveGRAS基因在冷、热和GA（3）反应中的不同功能。这项研究为深入了解FveGRAS基因在林地草莓中的潜在功能提供了依据。FveGRAS基因在匍匐茎形成、果实成熟和非生物胁迫等方面的功能有待进一步研究。
The cultivated strawberry (Fragaria x ananassa), an allo-octoploid with non-climacteric fleshy fruits, is a popular Rosaceae horticultural crop worldwide that is mainly propagated via stolons during cultivation. Woodland strawberry (Fragaria vesca), one of the four diploid progenitor species of cultivated strawberry, is widely used as a model plant in the study of Rosaceae fruit trees, non-climacteric fruits and stolons. One GRAS transcription factor has been shown to regulate stolon formation; the other GRAS proteins in woodland strawberry remain unknown. In this study, we identified 54 FveGRAS proteins in woodland strawberry, and divided them into 14 subfamilies. Conserved motif analysis revealed that the motif composition of FveGRAS proteins was conserved within each subfamily, but diverged widely among subfamilies. We found 56 orthologous pairs of GRAS proteins between woodland strawberry and Arabidopsis thaliana, 47 orthologous pairs between woodland strawberry and rice and 92 paralogous pairs within woodland strawberry. The expression patterns of FveGRAS genes in various organs and tissues, and changes therein under cold, heat and GA(3) treatments, were characterized using transcriptomic analysis. The results showed that 34 FveGRAS genes were expressed with different degrees in at least four organs, including stolons; only a few genes displayed organ-specific expression. The expression levels of 16 genes decreased, while that of four genes increased during fruit ripening; FveGRAS54 showed the largest increase in expression. Under cold, heat and GA(3) treatments, around half of the FveGRAS genes displayed increased or decreased expression to some extent, suggesting differing functions of these FveGRAS genes in the responses to cold, heat and GAs. This study provides insight into the potential functions of FveGRAS genes in woodland strawberry. A few FveGRAS genes were identified as candidate genes for further study, in terms of their functions in stolon formation, fruit ripening and abiotic stresses.