Upregulation of plasma C9 protein in gastric cancer patients.
Upregulation of plasma C9 protein in gastric cancer patients.
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DOI：
10.1002/pmic.201000127
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发表时间：
2010-09
期刊：
影响因子：
3.4
通讯作者：
Lim, Yoon Pin
中科院分区：
文献类型：
--
作者：
Chong, Poh-Kuan;Lee, Huiyin;Loh, Marie Chiew Shia;Choong, Lee-Yee;Lin, Qingsong;So, Jimmy Bok Yan;Lim, Khong Hee;Soo, Ross Andrew;Yong, Wei Peng;Chan, Siew Pang;Smoot, Duane T.;Ashktorab, Hassan;Yeoh, Khay Guan;Lim, Yoon Pin
胃癌是全球癌症相关死亡的主要原因之一。目前临床上使用的生物标志物对检测胃癌的敏感性不高。为了发现新的更好的生物标志物，用iTRAQ-LC-MS/MS方法对25例正常、15例早期和21例晚期癌症的血浆样本进行了蛋白质谱分析。胃癌组C9蛋白水平明显高于正常对照组。免疫印迹数据显示了与iTRAQ结果一致的趋势。C9在正常状态和癌症状态之间的区分能力不是由于患者之间的差异，而是独立于患者的胃炎和幽门螺杆菌状态。与正常细胞相比，在一组胃癌细胞系及其条件培养液中也可以检测到C9的过度表达，这意味着癌症患者血浆中C9水平的升高可能与胃癌的存在有关。随后对总共119份血浆样本进行的盲法试验研究表明，C9的敏感性可高达90%，特异性为74%。因此，C9是一种潜在的检测胃癌的生物标志物。
Gastric cancer is one of the leading causes of cancer-related deaths worldwide. Current biomarkers used in the clinic do not have sufficient sensitivity for gastric cancer detection. To discover new and better biomarkers, protein profiling on plasma samples from 25 normal, 15 early-stage and 21 late-stage cancer was performed using an iTRAQ-LC-MS/MS approach. The level of C9 protein was found to be significantly higher in gastric cancer compared with normal subjects. Immunoblotting data revealed a congruent trend with iTRAQ results. The discriminatory power of C9 between normal and cancer states was not due to inter-patient variations and was independent from gastritis and Helicobacter pylori status of the patients. C9 overexpression could also be detected in a panel of gastric cancer cell lines and their conditioned media compared with normal cells, implying that higher C9 levels in plasma of cancer patients could be attributed to the presence of gastric tumor. A subsequent blind test study on a total of 119 plasma samples showed that the sensitivity of C9 could be as high as 90% at a specificity of 74%. Hence, C9 is a potentially useful biomarker for gastric cancer detection.
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DOI：
10.1093/jnci/djp058
发表时间：
2009-04-15
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
Komuro A;Yashiro M;Iwata C;Morishita Y;Johansson E;Matsumoto Y;Watanabe A;Aburatani H;Miyoshi H;Kiyono K;Shirai YT;Suzuki HI;Hirakawa K;Kano MR;Miyazono K
通讯作者：
Miyazono K
影响因子：
4.6
作者：
Greenstein, JD;Peake, PW;Charlesworth, JA
通讯作者：
Charlesworth, JA
影响因子：
4.4
作者：
Gan, Chee Sian;Chong, Poh Kuan;Wright, Phillip C.
通讯作者：
Wright, Phillip C.
影响因子：
3.4
作者：
Hamler, RL;Zhu, K;Lubman, DM
通讯作者：
Lubman, DM
影响因子：
4.4
作者：
Ho, Jiapei;Kong, Jacklyn-Wai-Fun;Lim, Yoon-Pin
通讯作者：
Lim, Yoon-Pin