Impact of frequent Bcl-2 expression on better prognosis in renal cell carcinoma patients
Impact of frequent Bcl-2 expression on better prognosis in renal cell carcinoma patients
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DOI：
10.1038/sj.bjc.6601454
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发表时间：
2004-01-01
影响因子：
8.8
通讯作者：
Tomita, F
中科院分区：
文献类型：
--
作者：
Itoi, T;Yamana, K;Tomita, F
以前，我们报道Bcl-2在肾细胞癌（RCC）标本中频繁表达，但p53突变是一个罕见的事件。但Bcl-2阳性表达与肾癌的临床病理特征及预后的关系尚不清楚。因此，我们研究了Bcl-2蛋白在101例肾细胞癌中的表达及其作用。检测细胞增殖指数（PI）、凋亡指数（AI）、caspase-3和p53蛋白表达。免疫组化法检测Bcl-2、caspase-3和p53蛋白表达。为了研究肿瘤细胞的增殖活性和凋亡，分别基于Ki-67和末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记（TUNEL）阳性细胞计算PI和AI。Bcl-2蛋白阳性表达率为71.3%（72/101）。Bcl-2阳性表达与PI呈负相关（P
Previously, we reported that Bcl-2 was frequently expressed in renal cell carcinoma (RCC) specimens, but p53 mutation was a rare event. However, it was unclear whether Bcl-2 positivity was associated with the clinicopathological characteristics and prognosis in RCC. Therefore, we investigated the expression of Bcl-2 protein and its roles in 101 RCC specimens. In addition, the proliferation index (PI), apoptotic index (AI), caspase-3 and p53 expression were examined. The immunohistochemical method was applied for Bcl-2, caspase-3 and p53 protein expression. To investigate the proliferation activity and apoptosis of tumour cells, PI and AI were calculated based on Ki-67 and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labelling (TUNEL)-positive cells, respectively. Bcl-2 expression was detected in 72 out of 101 (71.3%) specimens. Bcl-2 positivity was inversely correlated with PI (P