The role of oxyR and soxRS in oxidative stress survival in Shigella flexneri.
The role of oxyR and soxRS in oxidative stress survival in Shigella flexneri.
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DOI：
10.1016/j.micres.2011.09.004
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发表时间：
2012-04-20
影响因子：
6.7
通讯作者：
Runyen-Janecky, Laura
中科院分区：
文献类型：
--
作者：
Daugherty, Aaron;Suvarnapunya, Akamol Eddie;Runyen-Janecky, Laura
福氏志贺菌是一种兼性胞内致病菌，暴露于人体内外的各种环境中。这些环境中的一些可能含有显著的氧化应激。S.产生在主要氧化应激调节剂oxyR和/或soxRS中缺失的福氏突变体，以测试它们在志贺氏菌生物学中的重要性。含有oxyR缺失的菌株在需氧生长过程中生长和存活减少，但微需氧生长没有减少。突变体也有缺陷的生存暴露于氧化应激：oxyR突变体是敏感的过氧化氢，而soxRS突变体是敏感的超氧化物。尽管ΔsoxRS、ΔoxyR和ΔoxyR/ΔsoxRS突变志贺氏菌在巨噬细胞内的存活与亲本菌株相似，但突变体在Henle细胞单层上形成的噬斑略小于野生型菌株形成的噬斑。
Shigella flexneri, a facultative intracellular pathogen, is exposed to a variety of environments inside and outside of the human host. Some of these environments may contain significant oxidative stress. S. flexneri mutants were generated with deletions in the major oxidative stress regulators oxyR and/or soxRS to test their importance in Shigella biology. Strains that contained a deletion of oxyR had reduced growth and survival during aerobic growth, but not microaerobic growth. The mutants were also defective in surviving exposure to oxidative stress: oxyR mutants were sensitive to hydrogen peroxide, while soxRS mutants were sensitive to superoxide. Although the ΔsoxRS, ΔoxyR, and ΔoxyR/ΔsoxRS mutant Shigellae survived similarly to the parental strains within macrophages, the mutants formed plaques on Henle cell monolayers that were slightly smaller than the plaques formed by the wildtype strain.
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影响因子：
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