In vitro effect of subinhibitory concentrations of antibiotics on biofilm formation by clinical strains of Salmonella enterica serovar Typhimurium isolated in Slovakia
In vitro effect of subinhibitory concentrations of antibiotics on biofilm formation by clinical strains of Salmonella enterica serovar Typhimurium isolated in Slovakia
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DOI：
10.1111/j.1365-2672.2007.03653.x
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发表时间：
2008-05-01
影响因子：
4
通讯作者：
Majtan, V.
中科院分区：
文献类型：
--
作者：
Majtan, J.;Majtanova, L.;Majtan, V.
目的：在本研究中，我们检测了 75 株鼠伤寒沙门氏菌 (Salm. Typhimurium) 人类临床分离株的生物膜形成以及庆大霉素、环丙沙星和头孢噻肟的亚抑制浓度 (sub-MIC) 对生物膜形成和胞外多糖 (EPS) 产生的影响。方法和结果： 分别使用改进的微量滴定板测定法和分光光度法进行生物膜形成和EPS生产。结果表明，以 DT104 噬菌体类型为主的 38 个分离株（50.7%）在体外是塑料表面强生物膜产生者。当具有最高生物膜形成能力的菌株在庆大霉素和环丙沙星的亚 MIC 存在下生长时，观察到生物膜形成和 EPS 产生受到抑制。相比之下，1/2 MIC (0.039 mu g ml(-1)) 的头孢噻肟能够显着诱导分别具有不可分型和 DT104 噬菌体类型的三个分离株生物膜和 EPS 的产生。结论：这些结果清楚地表明，所有测试的三种抗生素都能够干扰 Salm 的生物膜形成和 EPS 产生。鼠伤寒分离株。研究的意义和影响：当前的研究表明，亚 MIC 的头孢噻肟可能对病原体 Salm 的行为有益。鼠伤寒体外。
Aims: In this study, we examined the biofilm formation of 75 Salmonella enterica serovar Typhimurium (Salm. Typhimurium) human clinical isolates and the effect of subinhibitory concentrations (sub-MICs) of gentamicin, ciprofloxacin and cefotaxime on biofilm formation and exopolysaccharides (EPS) production.Methods and Results: Quantification of biofilm formation and EPS production were carried out using a modified microtitre plate assay and spectrophotometric method, respectively. The results indicate that 38 isolates (50.7%), which are predominantly of DT104 phage type, presented as the strong biofilm producers in vitro on plastic surface. When strains with the highest biofilm-forming capacity were grown in the presence of sub-MICs of gentamicin and ciprofloxacin, the inhibition of biofilm formation and EPS production was observed. In contrast, cefotaxime at 1/2 MIC (0.039 mu g ml(-1)) was able to significantly induce the production of biofilm as well as EPS in three isolates with nontypable and DT104 phage type, respectively.Conclusions: These results clearly indicate that all the three antibiotics tested are able to interfere with biofilm formation and EPS production by Salm. Typhimurium isolates.Significance and Impact of the Study: The current study demonstrated that cefotaxime at sub-MIC can be beneficial for the behaviour of pathogen Salm. Typhimurium in vitro.