Relationship between the ATP content measured at three imbibition times and germination of onion seeds during storage at 3, 15 and 30°C
Relationship between the ATP content measured at three imbibition times and germination of onion seeds during storage at 3, 15 and 30°C
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洋葱种子在 3、15 和 30°C 储存期间三个吸胀时间测量的 ATP 含量与发芽率之间的关系
DOI：
10.1093/jxb/45.10.1365
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发表时间：
1994
影响因子：
6.9
通讯作者：
V. Douet
中科院分区：
文献类型：
--
作者：
P. Siegenthaler;V. Douet
以洋葱（Allium cepa cv）为研究对象，研究了ATP含量作为种子品质指标的可能性。Wadenswil)。在3℃、15℃和30℃三种温度下，比较了145周内吸收种子的发芽率和ATP含量。在风干种子中检测不到的ATP含量（水分含量为9%，w/w）随着吸胀时间的增加而迅速增加，鲜重和呼吸速率也随之增加，在约17小时后达到稳定水平。贮藏36周后，3℃贮藏的种子的ATP形成率高于15℃和30℃贮藏的种子。此外，ATP合成的开始被延迟。这些现象很可能是种子老化的一种表现，是有用的指标，可以在储存36周后发芽率下降之前预测种子活力的丧失。此外，当洋葱种子的吸胀时间为17 h时，在145周的贮藏时间内，ATP含量与种子萌发能力之间的相关性非常好（15℃时r=0.95, 30℃时r= 0.97）。本文就ATP含量与种子发芽率相关性的争议进行了讨论。
The possibility of using ATP content as an indicator of seed quality was studied in onion seeds {Allium cepa cv. Wadenswil). The percentage germination and ATP content of imbibed seeds were compared during 145 weeks of storage at three temperatures (3, 15 and 30 °C). ATP content, which was undetectable in air-dried seeds (moisture content: 9%, w/w), increased rapidly as a function of imbibition time, as did the fresh weight and respiration rate, reaching a steady-state level after about 17 h. After 36 weeks of storage, the rate of ATP formation was greater for the seeds stored at 3°C than for those kept at 15 and 30 °C. Furthermore, the onset of ATP synthesis was delayed. These phenomena, which are likely to be an expression of seed ageing, are useful indicators, allowing the prediction of the loss of seed viability before the decrease in percentage germination which occurred beyond 36 weeks of storage. In addition, the correlation between ATP content and germination capacity of seeds during 145 weeks of storage was excellent (r=0.95 at 15°C and 0.97 at 30 °C), provided that a 17 h-imbibition time, specific for onion seeds, was chosen. These results are discussed in terms of the controversy concerning the correlation between the ATP content and germination percentage of seeds.