Identification and mapping of a tiller inhibition gene (tin3) in wheat
Identification and mapping of a tiller inhibition gene (tin3) in wheat
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DOI：
10.1007/s00122-006-0431-y
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发表时间：
2007-01-01
影响因子：
5.4
通讯作者：
Gill, Bikram S.
中科院分区：
文献类型：
--
作者：
Kuraparthy, Vasu;Sood, Shilpa;Gill, Bikram S.
分蘖是谷类作物中最重要的农艺性状之一，因为每株植物的分蘖数决定了每株植物的穗或穗的数量，这是谷物产量和/或生物量的关键组成部分。为了表征分蘖的潜在遗传变异，我们使用基于甲基磺酸乙酯（EMS）的诱变在二倍体小麦（Triticum monococcum subsp.单球菌）。分蘖突变体，分蘖抑制（tin 3）只产生一个主茎相比，野生型与许多分蘖。tin 3的单茎表型是由于一个单一的隐性突变。通过对二倍体小麦F-2群体的遗传和分子定位，将tin 3基因定位在染色体3A（m）的长臂上。在F-2群体中，共显性RFLP标记Xpsr 1205与tin 3共分离。利用PSR 1205对中国春3号染色体缺失系进行物理定位，将tin 3基因定位在小麦3A染色体长臂的远端10%，这是小麦重组富集区。锡3染色体臂3A（m）上的映射的影响进行了讨论，在3L共线区域跨各种谷物基因组和其他分蘖性状的草的锡3推定的直系同源物。
Tillering is one of the most important agronomic traits in cereal crops because tiller number per plant determines the number of spikes or panicles per plant, a key component of grain yield and/or biomass. In order to characterize the underlying genetic variation for tillering, we have isolated mutants that are compromised in tillering ability using ethyl methanesulphonate (EMS)-based mutagenesis in diploid wheat (Triticum monococcum subsp. monococcum). The tillering mutant, tiller inhibition (tin3) produces only one main culm compared to the wild type with many tillers. The monoculm phenotype of tin3 is due to a single recessive mutation. Genetic and molecular mapping in an F-2 population of diploid wheat located the tin3 gene on the long arm of chromosome 3A(m). One codominant RFLP marker Xpsr1205 cosegregated with tin3 in the F-2 population. Physical mapping of PSR1205 in a set of Chinese Spring deletion lines of group-3 chromosomes placed the tin3 gene in the distal 10% of the long arm of chromosome 3A, which is a recombination-rich region in wheat. The implications of the mapping of tin3 on chromosome arm 3A(m) are discussed with respect to putative orthologs of tin3 in the 3L colinear regions across various cereal genomes and other tillering traits in grasses.