A Catalog of Proteins Expressed in the AG Secreted Fluid during the Mature Phase of the Chinese Mitten Crabs (Eriocheir sinensis).
A Catalog of Proteins Expressed in the AG Secreted Fluid during the Mature Phase of the Chinese Mitten Crabs (Eriocheir sinensis).
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中华绒螯蟹成熟期AG分泌液表达的蛋白质目录
DOI：
10.1371/journal.pone.0136266
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Wang Q
中科院分区：
文献类型：
--
作者：
He L;Li Q;Liu L;Wang Y;Xie J;Yang H;Wang Q
副腺（AG）是节肢动物雄性生殖系统的重要组成部分，其分泌物可提高生殖能力，一些AG蛋白与精子结合，影响精子的功能和性质。本文报道了中华绒螯蟹（Eriocheir sinensis）成熟期AG分泌液的第一个综合目录。AG蛋白采用一维凝胶电泳分离，反相高效液相色谱-串联质谱（HPLC-MS/MS）分析。总共检测了1173个多肽的质谱（其中1067个没有诱饵和污染物），从而在NCBI数据库（http://www.ncbi.nlm.nih.gov/）和转录组数据集中鉴定了486种不同的蛋白质。质谱蛋白质组学数据已在ProteomeXchange上保存，标识符为PXD000700。AG蛋白质组的广泛描述将有助于为更好地理解一些生殖机制提供基础，包括潜在的精包破裂，精子细胞的动态功能和形态变化以及精子顶酶活力。因此，本文提供的蛋白质综合目录可为今后研究甲壳类动物生殖过程中精子成熟和调控机制提供有价值的参考。
The accessory gland (AG) is an important component of the male reproductive system of arthropods, its secretions enhance fertility, some AG proteins bind to the spermatozoa and affect its function and properties. Here we report the first comprehensive catalog of the AG secreted fluid during the mature phase of the Chinese mitten crab (Eriocheir sinensis). AG proteins were separated by one-dimensional gel electrophoresis and analyzed by reverse phase high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). Altogether, the mass spectra of 1173 peptides were detected (1067 without decoy and contaminants) which allowed for the identification of 486 different proteins annotated upon the NCBI database (http://www.ncbi.nlm.nih.gov/) and our transcritptome dataset. The mass spectrometry proteomics data have been deposited at the ProteomeXchange with identifier PXD000700. An extensive description of the AG proteome will help provide the basis for a better understanding of a number of reproductive mechanisms, including potentially spermatophore breakdown, dynamic functional and morphological changes in sperm cells and sperm acrosin enzyme vitality. Thus, the comprehensive catalog of proteins presented here can serve as a valuable reference for future studies of sperm maturation and regulatory mechanisms involved in crustacean reproduction.