The evolution of Sox gene repertoires and regulation of segmentation in arachnids
The evolution of Sox gene repertoires and regulation of segmentation in arachnids
复制标题
蛛形纲动物 Sox 基因库的进化和节段调控
DOI：
10.1101/2020.06.04.133389
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Baudouin-Gonzalez L
中科院分区：
文献类型：
--
作者：
Baudouin-Gonzalez L
SOX转录因子家族在后生动物发育过程中调节许多过程，包括干细胞维持和神经系统规范。因此，表征这些基因的谱系和作用可以为动物的进化和发育提供重要的见解。我们进一步描述了具有和不具有祖先全基因组重复的几个蜘蛛物种的SOX谱系，并比较了它们在蜘蛛Parasteatoda tepidariorum和收获的Phalangium opilio之间的表达。我们发现，大多数SOX家族在全基因组复制和潜在的亚功能化和/或新功能化事件的证据后被保留为同源基因。我们的结果还表明，Sox21b-1可能在蜘蛛类中调节祖先的片段，发挥着与昆虫中密切相关的SoxB基因Dichaete相似的作用。我们先前已经证明Sox21b-1是同时形成前体节和后体节的顺序添加所必需的。我们进一步研究了Sox21b-1基因在该蜘蛛中的表达和功能，发现虽然该基因调控着前体节和异体节段的产生，但它在这些标记物的形成中扮演着不同的角色，反映了它们不同的分割模式和不同结构的基因调控网络的部署。
The Sox family of transcription factors regulates many processes during metazoan development, including stem cell maintenance and nervous system specification. Characterizing the repertoires and roles of these genes can therefore provide important insights into animal evolution and development. We further characterized the Sox repertoires of several arachnid species with and without an ancestral whole-genome duplication and compared their expression between the spiderParasteatoda tepidariorumand the harvestmanPhalangium opilio. We found that most Sox families have been retained as ohnologs after whole-genome duplication and evidence for potential subfunctionalization and/or neofunctionalization events. Our results also suggest thatSox21b-1likely regulated segmentation ancestrally in arachnids, playing a similar role to the closely related SoxB gene,Dichaete, in insects. We previously showed thatSox21b-1is required for the simultaneous formation of prosomal segments and sequential addition of opisthosomal segments inP. tepidariorum. We studied the expression and function ofSox21b-1further in this spider and found that although this gene regulates the generation of both prosomal and opisthosomal segments, it plays different roles in the formation of these tagmata reflecting their contrasting modes of segmentation and deployment of gene regulatory networks with different architectures.