Near-infrared autofluorescence for the detection of parathyroid glands
Near-infrared autofluorescence for the detection of parathyroid glands
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DOI：
10.1117/1.3583571
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发表时间：
2011-06-01
影响因子：
3.5
通讯作者：
Mahadevan-Jansen, Anita
中科院分区：
文献类型：
--
作者：
Paras, Constantine;Keller, Matthew;Mahadevan-Jansen, Anita
内分泌外科的一个主要挑战是在甲状腺切除术和甲状旁腺切除术中术中检测甲状旁腺。目前的定位技术，如超声和sestamibi扫描大多在术前，并依赖于异常甲状旁腺的检测。在本文中，我们提出了近红外（NIR）自体荧光作为一种非侵入性的，实时的，自动的体内检测甲状旁腺的方法。进行了一项试点体内研究，以评估近红外荧光在甲状腺和甲状旁腺切除术期间识别甲状旁腺的能力。对21例接受内分泌手术的患者，术中对颈部暴露的不同组织进行785 nm激发下的荧光测量。发现甲状旁腺的荧光强度始终大于甲状腺和颈部所有其他组织的荧光强度。特别是甲状旁腺的荧光比甲状腺组织高2 ~ 11倍，荧光峰值出现在820 ~ 830 nm。这些结果表明，近红外荧光有潜力成为一个优秀的光学工具，定位甲状旁腺组织在手术中。(C) 2011光学仪器工程师学会（SPIE）(DOI: 10.1117/1.3583571)
A major challenge in endocrine surgery is the intraoperative detection of parathyroid glands during both thyroidectomies and parathyroidectomies. Current localization techniques such as ultrasound and sestamibi scan are mostly preoperative and rely on an abnormal parathyroid for its detection. In this paper, we present near-infrared (NIR) autofluorescence as a nonintrusive, real-time, automated in vivo method for the detection of the parathyroid gland. A pilot in vivo study was conducted to assess the ability of NIR fluorescence to identify parathyroid glands during thyroid and parathyroidectomies. Fluorescence measurements at 785 nm excitation were obtained intra-operatively from the different tissues exposed in the neck region in 21 patients undergoing endocrine surgery. The fluorescence intensity of the parathyroid gland was found to be consistently greater than that of the thyroid and all other tissues in the neck of all patients. In particular, parathyroid fluorescence was two to eleven times higher than that of the thyroid tissues with peak fluorescence occurring at 820 to 830 nm. These results indicate that NIR fluorescence has the potential to be an excellent optical tool to locate parathyroid tissue during surgery. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI:10.1117/1.3583571]