Brain atrophy in a murine model of chronic fatigue syndrome and beneficial effect of Hochu-ekki-to (TJ-41)
Brain atrophy in a murine model of chronic fatigue syndrome and beneficial effect of Hochu-ekki-to (TJ-41)
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DOI：
10.1007/s11064-008-9620-1
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发表时间：
2008-09-01
影响因子：
4.4
通讯作者：
Kanda, Tsugiyasu
中科院分区：
文献类型：
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作者：
Chen, Rui;Moriya, Junji;Kanda, Tsugiyasu
脑源性神经营养因子（BDNF）与慢性疲劳综合征（CFS）的主要症状和神经元凋亡有关。然而，还没有直接研究CFS和BDNF与神经元凋亡的关系。我们在BALB/c小鼠中通过六次注射灭活的牛种布鲁氏菌抗原诱导CFS模型，并用Hochu-ekki-to（TJ-41）治疗它们。测定大鼠每日跑步活动量、体重、脑重/体重（CW/BW）及海马BDNF和Bcl-2 mRNA表达水平。CFS模型组大鼠的日活动量和体重/体重显著降低。CFS模型组和TJ-41治疗组小鼠海马BDNF和Bcl-2 mRNA表达水平均受到抑制，但两者之间无显著性差异。我们改进了一种小鼠模型，以探讨CFS与脑功能障碍的关系。在该模型中，日常活动减少可能与海马BDNF mRNA表达减少、海马细胞凋亡和脑萎缩相关。TJ-41增加模型的日常跑步活动，这与脑恢复无关。
Brain-derived neurotrophic factor (BDNF) is associated with the main symptoms of chronic fatigue sydrome (CFS) and neuron apoptosis. Nevertheless, no study has been performed directly to explore the relationship between CFS, BDNF and neuron apoptosis. We induced a CFS model by six injections of killed Brucella abortus antigen in BALB/c mice and treated them with Hochu-ekki-to (TJ-41). Daily running activity, body weight (BW), ratio of cerebral weight to BW (CW/BW) and expression levels of BDNF and Bcl-2 mRNA in the hippocampus were determined. The daily activity and CW/BW decreased significantly in the CFS model. BDNF and Bcl-2 mRNA expression levels in the hippocampus were suppressed in the CFS model and TJ-41 treated mice, while no significant difference was found between them. We improved a murine model to investigate the relationship between CFS and brain dysfunction. In this model, reduced daily activity might have been associated with decreased hippocampal BDNF mRNA expression, hippocampal apoptosis and brain atrophy. TJ-41 increased the daily running activity of the model, which was independent of brain recovery.