Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila.
Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila.
复制标题
从嗜肺军团菌中分离 Dot/Icm IV 型分泌系统核心复合物。
DOI：
10.1007/978-1-4939-9048-1_15
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Nagai H.
中科院分区：
文献类型：
--
作者：
Kubori T;Nagai H.
Legionellautilizes specialized protein secretion machinery called the type IV secretion system encoded bydot/icmgenes to modulate host cellular systems. We describe here the procedure to isolate the core complex of the Dot/Icm type IV secretion system ofL. pneumophilabased on detergent lysis of bacteria and ultracentrifugation. The isolated protein complex can be applied for biochemical and transmission electron microscopy analysis.
DOI：
10.1073/pnas.1404506111
发表时间：
2014-08-12
影响因子：
11.1
作者：
Kubori, Tomoko;Koike, Masafumi;Nagai, Hiroki
通讯作者：
Nagai, Hiroki