APeg3: regulation of Peg3 through an evolutionarily conserved ncRNA.
APeg3: regulation of Peg3 through an evolutionarily conserved ncRNA.
复制标题
APeg3：通过进化上保守的 ncRNA 调节 Peg3。
DOI：
10.1016/j.gene.2014.02.056
复制
发表时间：
2014
期刊：
影响因子：
3.5
通讯作者：
Kim,Joomyeong
中科院分区：
文献类型：
--
作者：
Frey,WesleyD;Kim,Joomyeong
哺乳动物APeg3是一种反义基因，位于印记基因Peg3的3'非翻译区域内。APeg3仅在下丘脑的加压素神经元中表达，因此预计在大脑的这一特定区域中发挥重要作用。在当前的研究中，我们通过比较基因组学和基于细胞系的功能方法研究了 APeg3 的功能。 APeg3的转录区在胎盘哺乳动物中表现出高度的序列保守性，但没有任何明显的开放阅读框，表明APeg3可能在真兽类进化过程中被选择作为ncRNA基因。 APeg3 内保守的局部 RNA 二级结构的检测进一步支持了这一点。 RNA 二级结构分析表明转录本 5' 端有一个保守的发夹环结构。基于细胞系的转染实验结果表明APeg3具有下调Peg3转录和蛋白水平的潜力。观察到的 APeg3 下调在某种程度上也与方向无关。总体而言，这些结果表明 APeg3 已进化为 ncRNA 基因，并在特定神经元细胞内控制其正义基因 Peg3 的功能。
MammalianAPeg3is an antisense gene that is localized within the 3′-untranslated region of the imprinted gene,Peg3.APeg3is expressed only in the vasopressinergic neurons of the hypothalamus, thus is predicted to play significant roles in this specific area of the brain. In the current study, we investigate the functions ofAPeg3with comparative genomics and cell line-based functional approaches. The transcribed region ofAPeg3displays high levels of sequence conservation among placental mammals, but without any obvious open reading frame, suggesting thatAPeg3may have been selected as a ncRNA gene during eutherian evolution. This has been further supported by the detection of a conserved local RNA secondary structure withinAPeg3. RNA secondary structure analyses indicate a single conserved hairpin-loop structure towards the 5′ end of the transcript. The results from cell line-based transfection experiments demonstrate thatAPeg3has the potential to down-regulate the transcription and protein levels ofPeg3. The observed down-regulation byAPeg3is also somewhat orientation-independent. Overall, these results suggest thatAPeg3has evolved as a ncRNA gene and controls the function of its sense genePeg3within specific neuronal cells.