A primer on resolving the nanoscale structure of the plasma membrane with light and electron microscopy.
A primer on resolving the nanoscale structure of the plasma membrane with light and electron microscopy.
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用光学和电子显微镜解析质膜纳米级结构的入门读物。
DOI：
10.1085/jgp.201812227
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Taraska,JustinW
中科院分区：
文献类型：
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作者：
Taraska,JustinW
质膜将细胞与外界环境隔开。所有进入或离开细胞的物质和信号都必须穿过这个疏水屏障。了解质膜的结构和动力学一直是一般细胞生理学的中心焦点。光学显微镜和电子显微镜都是这项奋进的基础，并已被用来揭示质膜的致密，复杂和动态的纳米尺度景观。在这里，我回顾经典和最近的发展，用于图像和研究质膜的结构，特别是光，电子和相关的显微镜的方法。我将讨论它们的历史和用于绘制质膜的用途，并重点介绍这些工具如何为在分子尺度上理解膜提供结构框架。最后，我将描述这些研究如何为确定其他细胞器和整个细胞的纳米级结构提供路线图，以弥合细胞形式和功能之间的差距。
The plasma membrane separates a cell from its external environment. All materials and signals that enter or leave the cell must cross this hydrophobic barrier. Understanding the architecture and dynamics of the plasma membrane has been a central focus of general cellular physiology. Both light and electron microscopy have been fundamental in this endeavor and have been used to reveal the dense, complex, and dynamic nanoscale landscape of the plasma membrane. Here, I review classic and recent developments in the methods used to image and study the structure of the plasma membrane, particularly light, electron, and correlative microscopies. I will discuss their history and use for mapping the plasma membrane and focus on how these tools have provided a structural framework for understanding the membrane at the scale of molecules. Finally, I will describe how these studies provide a roadmap for determining the nanoscale architecture of other organelles and entire cells in order to bridge the gap between cellular form and function.