Infection of human cells by an endogenous retrovirus of pigs
Infection of human cells by an endogenous retrovirus of pigs
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DOI：
10.1038/nm0397-282
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发表时间：
1997-03-01
期刊：
影响因子：
82.9
通讯作者：
Weiss, RA
中科院分区：
文献类型：
--
作者：
Patience, C;Takeuchi, Y;Weiss, RA
在生物医学研究中，正在积极考虑将猪的器官和组织用作人类异种移植的可能性。因此，我们研究了猪内源性逆转录病毒(PERV)基因组能否在培养中感染人类细胞。两个猪肾细胞系自发产生C型逆转录病毒颗粒。由PERV-15肾细胞系(PERV-PK)感染猪、水貂和人肾293细胞产生的无细胞逆转录病毒，以及经X-射线照射的PK-15细胞与人细胞共培养，导致更广泛的人类细胞感染，包括人二倍体成纤维细胞以及B和T细胞系。家猪肾、心、脾组织中含有多个完整的PERV基因组拷贝，并表达病毒RNA。在人类细胞中传代后，PERV-PK可以拯救Moloney逆转录病毒载体，并获得对人类补体裂解的抵抗力。
The possible use of pig organs and tissues as xenografts in humans is actively being considered in biomedical research. We therefore examined whether pig endogenous retrovirus (PERV) genomes can be infectiously transmitted to human cells in culture. Two pig kidney cell lines spontaneously produce C-type retrovirus particles. Cell-free retrovirus produced by the PK-15 kidney cell line (PERV-PK) infected pig, mink and human kidney 293 cell lines and co-cultivation of X-irradiated PK-15 cells with human cells resulted in a broader range of human cell infection, including human diploid fibroblasts and B- and T-cell lines. Kidney, heart and spleen tissue obtained from domestic pigs contained multiple copies of integrated PERV genomes and expressed viral RNA. Upon passage in human cells PERV-PK could rescue a Moloney retroviral vector and acquired resistance to lysis by human complement.