Switchable hydrophilicity solvent membrane-based microextraction: HPLC-FLD determination of fluoroquinolones in shrimps.
Switchable hydrophilicity solvent membrane-based microextraction: HPLC-FLD determination of fluoroquinolones in shrimps.
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DOI：
10.1016/j.aca.2017.04.054
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发表时间：
2017-07
影响因子：
6.2
通讯作者：
A. Pochivalov;I. Timofeeva;Christina Vakh;A. Bulatov
中科院分区：
文献类型：
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作者：
A. Pochivalov;I. Timofeeva;Christina Vakh;A. Bulatov
提出了一种可切换亲水性溶剂膜基微萃取（SHS-MME）策略，用于复杂基质的简单、高可用性样品预处理。SHS- mme过程是基于将目标分析物从含水样品中提取到浸渍有可切换亲水性溶剂（SHS）的多孔疏水膜中，然后在碱性受体溶液中进行SHS电离和反萃取。研究了中链脂肪酸作为氟喹诺酮类药物（氟沙星、洛美沙星、诺氟沙星和氧氟沙星）SHS- mme的SHS。采用高效液相色谱法（HPLC-FLD）对虾类样品中氟喹诺酮类药物的含量进行了测定。在氧氟沙星3 ~ 1500 μg L−1、诺氟沙星10 ~ 1000 μg L−1、洛美沙星和氟罗沙星15 ~ 1500 μg L−1的浓度范围内，标定曲线呈线性关系。根据3σ计算空白检验的检出限：氧氟沙星为1 μg L−1，诺氟沙星为3 μg L−1，洛美沙星和氟罗沙星为5 μg L−1。SHS电离可以被认为是膜基液相微萃取分析应用中的一个新的潜在场。
A switchable hydrophilicity solvent membrane-based microextraction (SHS-MME) strategy for simple and highly-available sample pretreatment of complex matrices has been proposed. The SHS-MME procedure based on extraction of target analytes from an aqueous sample into a porous hydrophobic membrane impregnated with a switchable hydrophilicity solvent (SHS) followed by SHS ionization and back-extraction of the analytes in alkaline acceptor solution. The medium-chain fatty acids were investigated as SHS for the SHS-MME of fluoroquinolones (fleroxacin, lomefloxacin, norfloxacin and ofloxacin). The SHS-MME was successfully coupled with a HPLC-FLD for the determination of fluoroquinolones in shrimp samples with no further sample pretreatment. The calibration graphs were linear over the concentration ranges of 3–1500 μg L−1for ofloxacin, 10–1000 μg L−1for norfloxacin, 15–1500 μg L−1for lomefloxacin and fleroxacin. The limits of detection, calculated from a blank test based on 3σ, were 1 μg L−1for ofloxacin, 3 μg L−1for norfloxacin and 5 μg L−1for lomefloxacin and fleroxacin. The SHS ionization can be considered as new potential fields in analytical application of membrane-based liquid phase microextraction.