Identification of amplified and highly expressed genes in amplicons of the T-cell line huT78 detected by cDNA microarray CGH
Identification of amplified and highly expressed genes in amplicons of the T-cell line huT78 detected by cDNA microarray CGH
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DOI：
10.1186/1476-4598-4-5
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发表时间：
2005-01-18
期刊：
影响因子：
37.3
通讯作者：
Benitez, Javier
中科院分区：
文献类型：
--
作者：
Melendez, Barbara;Martinez-Delgado, Beatriz;Benitez, Javier
背景资料：常规比较基因组杂交（CGH）已被广泛用于检测癌症中的拷贝数改变和用于鉴定含有候选肿瘤责任基因的区域。最近，一些研究表明，cDNA微阵列CGH的效用，研究基因拷贝的变化，在各种类型的肿瘤。然而，尚未对T细胞淋巴瘤进行此类研究。到目前为止，通过使用染色体CGH分析的T细胞淋巴瘤仅显示出轻微的拷贝数改变，而不是基因扩增。在本研究中，我们描述了位于2q34-q37、8q23-q24和20p的T细胞系huT78的三个扩增子的表征，使用含有7.657个转录物的cDNA微阵列允许鉴定某些基因，例如BCLX、PCNA、FKBP 1A、IGFBP2和cMYC，其被扩增、高度表达，并且也包含在20 p和2q上的扩增子中。结论：利用常规CGH和CGH基因表达谱芯片，我们在huT78细胞中检测到3个扩增子，并发现了几个新的基因扩增产物（BCLX、PCNA、FKBP 1A、IGFBP2和cMYC）。我们发现，过度表达的扩增基因可以归因于基因剂量。我们推测这些基因的失调可能在T细胞淋巴瘤的发展和/或T细胞系的维持中是重要的。
Background: Conventional Comparative Genomic Hybridization ( CGH) has been widely used for detecting copy number alterations in cancer and for identifying regions containing candidate tumor responsible genes. Recently, several studies have shown the utility of cDNA microarray CGH for studing gene copy changes in various types of tumors. However, no such studies on T-cell lymphomas have been performed. To date T-cell lymphomas analyzed by the use of chromosome CGH have revealed only slight copy number alterations and not gene amplifications.Results: In the present study, we describe the characterization of three amplicons of the T-cell line huT78 located at 2q34-q37, 8q23-q24 and 20p, where new amplified and overexpressed genes are found. The use of a cDNA microarray containing 7.657 transcripts allowed the identification of certain genes, such as BCLX, PCNA, FKBP1A, IGFBP2 and cMYC, that are amplified, highly expressed, and also contained in the amplicons on 20p and 2q. The expresion of these genes was analyzed in 39 T-cell lymphomas and 3 other T-cell lines.Conclusion: By the use of conventional CGH and CGH and expression cDNA microarrays we defined three amplicons in the T-cell line huT78 and identified several novel gene amplifications ( BCLX, PCNA, FKBP1A, IGFBP2 and cMYC). We showed that overexpression of the amplified genes could be attributable to gene dosage. We speculate that deregulation of those genes could be important in the development of T-cell lymphomas and/or in the maintenance of T- cell lines.