Bacterioferritin comigratory protein is important in hydrogen peroxide resistance, nodulation, and nitrogen fixation in Azorhizobium caulinodans
Bacterioferritin comigratory protein is important in hydrogen peroxide resistance, nodulation, and nitrogen fixation in Azorhizobium caulinodans
复制标题
细菌铁蛋白共迁移蛋白在固氮根瘤菌的过氧化氢抗性、结瘤和固氮中发挥重要作用
DOI：
10.1007/s00203-019-01654-8
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发表时间：
2019-08-01
影响因子：
2.8
通讯作者：
Zhu, Jun
中科院分区：
文献类型：
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作者：
Liu, Xiaomeng;Qiu, Wei;Zhu, Jun
活性氧不仅对根瘤菌有害，而且是根瘤菌与豆科植物之间建立共生关系所必需的。在这项工作中，我们首先研究了细菌铁蛋白共迁移蛋白（BCP），过氧化物酶家族的成员，在固氮菌Azorhizobium caulinodans的初步作用。结果表明，bcp缺陷型A. caulinodans表现出增加的敏感性，无机过氧化氢（H2O2），但不是两个有机过氧化物的生长阶段依赖性的方式。同时，BCP被发现参与过氧化氢酶活性在相对较低的H2O2条件下。此外，bcp基因突变还显著影响了A.茎节类我们的工作最初记录了BCP在根瘤菌的自由生活阶段和它们与豆科植物的共生相互作用期间在细菌防御H2O2中的重要性。需要体内分子信号来破译A中BCP的整体功能。caulinodans以及其他根瘤菌。
Reactive oxygen species are not only harmful for rhizobia but also required for the establishment of symbiotic interactions between rhizobia and their legume hosts. In this work, we first investigated the preliminary role of the bacterioferritin comigratory protein (BCP), a member of the peroxiredoxin family, in the nitrogen-fixing bacterium Azorhizobium caulinodans. Our data revealed that the bcp-deficient strain of A. caulinodans displayed an increased sensitivity to inorganic hydrogen peroxide (H2O2) but not to two organic peroxides in a growth-phase-dependent manner. Meanwhile, BCP was found to be involved in catalase activity under relatively low H2O2conditions. Furthermore, nodulation and N2fixation were significantly impaired by mutation of the bcp gene in A. caulinodans. Our work initially documented the importance of BCP in the bacterial defence against H2O2in the free-living stage of rhizobia and during their symbiotic interactions with legumes. Molecular signalling in vivo is required to decipher the holistic functions of BCP in A. caulinodans as well as in other rhizobia.