Malignant melanoma. Interaction with coagulation and fibrinolysis pathways in situ.
Malignant melanoma. Interaction with coagulation and fibrinolysis pathways in situ.
复制标题
恶性黑色素瘤。
DOI：
10.1093/ajcp/93.4.516
复制
发表时间：
1990
影响因子：
3.5
通讯作者：
Stump,DC
中科院分区：
文献类型：
--
作者：
Wojtukiewicz,MZ;Zacharski,LR;Memoli,VA;Kisiel,W;Kudryk,BJ;Rousseau,SM;Stump,DC
应用于转移性恶性黑色素瘤组织的新鲜冷冻切片的免疫组化技术显示，在整个肿瘤结缔组织基质的血管周围区域中存在丰富的纤维蛋白原（或纤维蛋白I）。在存活肿瘤结节周围的结缔组织中容易检测到局灶性分布的纤维素。交联纤维蛋白的D-二聚体染色（使用与纤维蛋白原片段D交叉反应的抗体）与纤维蛋白染色一致。观察到肿瘤细胞体对因子X的弥漫染色，并且在整个肿瘤的结缔组织的分散区域中检测到因子XIII（“a”亚基）。未检测到因子VII，仅对罕见的肿瘤细胞进行组织因子染色。这些结果支持这样的概念，即肿瘤细胞相关的凝血酶生成途径原位存在于恶性黑色素瘤组织中，其包括因子X但既不包括组织因子也不包括因子VII。相比之下，尿激酶很少观察到肿瘤细胞染色，而组织型纤溶酶原激活剂的染色程度不同。
Immunohistochemical techniques applied to fresh frozen sections of metastatic malignant melanoma tissue revealed abundant fibrinogen (or fibrin I) in perivascular areas throughout the tumor connective tissue stroma. Fibrin was readily detected in a focal distribution in the connective tissue around nodules of viable tumor. Staining for D-dimer of cross-linked fibrin (using an antibody that cross-reacted with fragment D of fibrinogen) coincided with staining for fibrin. Diffuse staining of tumor cell bodies was observed for Factor X, and Factor XIII (“a” subunit) was detected in scattered areas of connective tissue throughout the tumors. Factor VII was not detected, and only rare tumor cells stained for tissue factor. These results support the concept that a tumor cell-associated, thrombin-generating pathway exists in situ in malignant melanoma tissue that includes Factor X but neither tissue factor nor Factor VII. By contrast, tumor cell staining was observed rarely for urokinase and to a variable extent for tissue plasminogen activator.