Lobe-related concentration- and Ca2+-dependent interactions of calmodulin with C- and N-terminal tails of the CaV1.2 channel
Lobe-related concentration- and Ca2+-dependent interactions of calmodulin with C- and N-terminal tails of the CaV1.2 channel
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DOI：
10.1007/s12576-013-0270-y
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发表时间：
2013-09-01
影响因子：
2.3
通讯作者：
Hao, Liying
中科院分区：
文献类型：
--
作者：
He, Guilin;Guo, Feng;Hao, Liying
本研究采用下拉分析法研究了钙调素（CaM）及其突变体与电压门控性Ca ~（2+）通道Ca（V）1.2在不同CaM和Ca ~（2+）浓度（[Ca ~（2+）]）下的C端和N端尾的结合，以获得关于结合模式的基本信息，包括其浓度和Ca ~（2+）依赖性。我们的数据表明，在高[Ca ~（2+）]时，不止一个CaM分子可以与Ca（V）1.2 C-末端尾结合。此外，CaM的C叶对于感知[Ca 2 +]与Ca（V）1.2的C末端尾部结合的变化非常关键，而CaM与Ca（V）1.2的N末端尾部之间的结合需要高[Ca 2 +]。我们的数据提供了钙调素和钙（V）1.2通道之间的相互作用的新细节。
This study examined the bindings of calmodulin (CaM) and its mutants with the C- and N-terminal tails of the voltage-gated Ca2+ channel Ca(V)1.2 at different CaM and Ca2+ concentrations ([Ca2+]) by using the pull-down assay method to obtain basic information on the binding mode, including its concentration- and Ca2+-dependencies. Our data show that more than one CaM molecule could bind to the Ca(V)1.2 C-terminal tail at high [Ca2+]. Additionally, the C-lobe of CaM is highly critical in sensing the change of [Ca2+] in its binding to the C-terminal tail of Ca(V)1.2, and the binding between CaM and the N-terminal tail of Ca(V)1.2 requires high [Ca2+]. Our data provide new details on the interactions between CaM and the Ca(V)1.2 channel.