CD46 is a cellular receptor for all species B adenoviruses except types 3 and 7
CD46 is a cellular receptor for all species B adenoviruses except types 3 and 7
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DOI：
10.1128/jvi.79.22.14429-14436.2005
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发表时间：
2005-11-01
影响因子：
5.4
通讯作者：
Arnberg, N
中科院分区：
文献类型：
--
作者：
Marttila, M;Persson, D;Arnberg, N
51个人腺病毒血清型分为6种(A到F)。除B种外，所有物种的腺病毒血清型都利用柯萨奇-腺病毒受体在体外附着到宿主细胞上。B类腺病毒主要引起眼部和呼吸道感染，但某些血清型也与肾脏疾病有关。我们以前已经证明，11型腺病毒(B种)使用CD46(膜辅助因子蛋白)作为细胞受体，而不是柯萨奇-腺病毒受体(A.Segerman等人，J.Virol)。77：9183-9191,2003)。在本研究中，我们发现，转染人CD46基因使低容许性中国仓鼠卵巢细胞对腺病毒3型和7型以外的所有B型腺病毒感染都有更强的容许性。此外，兔抗人CD46抗血清可以阻断或有效地抑制除腺病毒3型和7型外的所有B型腺病毒感染人A549细胞。我们还对50型腺病毒(B种)的纤维蛋白编码基因进行了测序，并将其与选定的血清型(包括B种的所有其他血清型)的相应氨基酸序列进行了比较。结果表明，CD46是除3型和7型外的所有B型腺病毒A549细胞上的主要细胞受体。
The 51 human adenovirus serotypes are divided into six species (A to F). Adenovirus serotypes from all species except species B utilize the coxsackie-adenovirus receptor for attachment to host cells in vitro. Species B adenoviruses primarily cause ocular and respiratory tract infections, but certain serotypes are also associated with renal disease. We have previously demonstrated that adenovirus type 11 (species B) uses CD46 (membrane cofactor protein) as a cellular receptor instead of the coxsackie-adenovirus receptor (A. Segerman et al., J. Virol. 77:9183-9191, 2003). In the present study, we found that transfection with human CD46 cDNA rendered poorly permissive Chinese hamster ovary cells more permissive to infection by all species B adenovirus serotypes except adenovirus types 3 and 7. Moreover, rabbit antiserum against human CD46 blocked or efficiently inhibited all species B serotypes except adenovirus types 3 and 7 from infecting human A549 cells. We also sequenced the gene encoding the fiber protein of adenovirus type 50 (species B) and compared it with the corresponding amino acid sequences from selected serotypes, including all other serotypes of species B. From the results obtained, we conclude that CD46 is a major cellular receptor on A549 cells for all species B adenoviruses except types 3 and 7.