Drosophila Rab14 mediates phagocytosis in the immune response to Staphylococcus aureus.
Drosophila Rab14 mediates phagocytosis in the immune response to Staphylococcus aureus.
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DOI：
10.1111/cmi.12220
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发表时间：
2014-02
影响因子：
3.4
通讯作者：
Wu LP
中科院分区：
文献类型：
--
作者：
Garg A;Wu LP
Drosophila hemocytes are essential for the animal to resist Staphylococcus aureus infections. Phagocytosis is a central component of the hemocyte-mediated immune response. It involves regulated interaction between the phagocytic and the endocytic compartments. Rab GTPases are pivotal for the membrane trafficking and fusion events, and thus are often targets of intracellular pathogens that subvert phagocytosis. An in vivo screen identified Rab2 and Rab14 as candidates for proteins regulating phagosome maturation. Since Rab14 is often targeted by intracellular pathogens, an understanding of its function during phagocytosis and the overall immune response can give insight into the pathogenesis of intracellular microbes. We generated a Drosophila Rab14 mutant and characterized the resulting immune defects in animals and specifically in hemocytes in response to an S. aureus infection. Hemocyte based immunofluorescence studies indicate that Rab14 is recruited to the phagosome and like Rab7, a well-characterized regulator of the phagocytic pathway, is essential for progression of phagosome maturation. Rab14 mutant hemocytes show impaired recruitment of Rab7 and of a lysosomal marker onto S. aureus phagosomes. The defect in phagocytosis is associated with higher bacterial load and increased susceptibility to S. aureus in the animal.
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影响因子：
3.5
作者：
Proikas-Cezanne, Tassula;Gaugel, Anja;Nordheim, Alfred
通讯作者：
Nordheim, Alfred
DOI：
10.1083/jcb.124.5.677
发表时间：
1994-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Desjardins M;Huber LA;Parton RG;Griffiths G
通讯作者：
Griffiths G
影响因子：
3.4
作者：
Duclos, Sophie;Clavarino, Giovanna;Desjardins, Michel
通讯作者：
Desjardins, Michel
影响因子：
5.3
作者：
Li, Q;Lau, A;Stanley, EF
通讯作者：
Stanley, EF
影响因子：
30.8
作者：
Parks, AL;Cook, KR;Francis-Lang, HL
通讯作者：
Francis-Lang, HL