Collagen type IV promotes the differentiation of neuronal progenitors and inhibits astroglial differentiation in cortical cell cultures
Collagen type IV promotes the differentiation of neuronal progenitors and inhibits astroglial differentiation in cortical cell cultures
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DOI：
10.1016/s0165-3806(98)00091-1
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发表时间：
1998-09-10
期刊：
影响因子：
--
通讯作者：
Parnavelas, JG
中科院分区：
文献类型：
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作者：
Ali, SA;Pappas, IS;Parnavelas, JG
为了阐明发育中的皮质细胞与其微环境之间的相互作用，我们采用分离的细胞培养和免疫细胞化学方法分析了在皮质生成期间，TV型胶原（COL）对大鼠皮质祖细胞增殖和分化的影响。COL存在于神经发生的整个时期的增殖区中，属于构成细胞外基质（ECM）的相当大部分的一组大分子蛋白质。我们已经表明，这种ECM分子抑制细胞增殖和神经胶质细胞分化，同时促进神经元分化。我们还证明，COL，当应用于与碱性成纤维细胞生长因子（bFGF）的文化，诱导神经胶质细胞分化，同时继续促进神经元分化。这些结果表明，皮质祖细胞对局部环境信号的反应不同，并且ECM的组分参与皮质生成的调节。(C)1998 Elsevier Science B.V.保留所有权利。
In an effort to elucidate the interactions between cells in the developing cortex and their microenvironment, we have employed dissociated cell cultures and immunocytochemistry to analyze the effect of collagen type TV (COL) on the proliferation and differentiation of rat cortical progenitor cells during the period of corticogenesis. COL, present in the proliferative zones throughout the period of neurogenesis, belongs to a group of macromolecular proteins that make up a considerable portion of the extracellular matrix (ECM). We have shown that this ECM molecule inhibits cell proliferation and glial cell differentiation while promoting neuronal differentiation. We have also demonstrated that COL, when applied to the cultures with basic fibroblast growth factor (bFGF), induces glial cell differentiation while continuing to promote neuronal differentiation. These results indicate that cortical progenitor cells respond differentially to local environmental signals, and that components of the ECM are involved in the regulation of corticogenesis. (C) 1998 Elsevier Science B.V. All rights reserved.