Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains in five provinces of China
Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains in five provinces of China
复制标题
DOI：
10.5588/ijtld.10.0403
复制
发表时间：
2011-06-01
影响因子：
4
通讯作者：
Song, C-X.
中科院分区：
文献类型：
--
作者：
Guo, Y-L.;Liu, Y.;Song, C-X.
目的：了解中国5省结核分枝杆菌北京型基因的分布情况及其与结核分支杆菌耐药株的相关性。设计：对中国地区5省分离的15 8株结核分枝杆菌进行6110限制性片段长度多态性插入序列分析、基因分型和结核分枝杆菌散布重复单位分析。测定并比较各省区北京型分离株的流行情况。用比例法进行药敏试验。结果：158株细菌中，有123株(77.8%)经RFLP和基因分型鉴定为北京型。几乎所有的菌株(n=152，96.2%)被分成14个共享的球型。其他六种种子型是中国所独有的。北京血型在内地的患病率显著高于沿海地区(P<；0.001，OR5.4，95%CI2.3-12.7)。北京株对利福平耐药与北京株相关(P<；0.05，OR 3.7，95%CI 1.2~11.1)。结论：中国地区不同地区结核分枝杆菌北京株的流行情况不同，仅与利福平耐药有关。
OBJECTIVE: To evaluate the distribution of the Mycobacterium tuberculosis Beijing genotype and the association of the genotype with drug-resistant M. tuberculosis strains in five provinces in China.DESIGN: M. tuberculosis strains (n = 158) isolated from five provinces of China were subjected to insertion sequence 6110 restriction fragment length polymorphism (RFLP), spoligotyping and mycobacterial interspersed repetitive units (MIRU) analyses. The prevalence of the Beijing genotype strains in each province was determined and compared. The proportion method was used to test the drug susceptibility of all strains.RESULT: Of the 158 strains, 123 (77.8%) were identified as the Beijing genotype by RFLP and spoligotyping. Nearly all the strains (n = 152, 96.2%) were grouped into 14 shared spoligotypes. Six other spoligotypes were unique to China. The prevalence of the Beijing genotype was significantly higher in the interior than in coastal areas (P < 0.001, OR 5.4, 95 %CI 2.3-12.7). Resistance to rifampicin (RMP) was associated with the Beijing strain (P < 0.05, OR 3.7, 95%CI 1.2-11.1).CONCLUSION: The M. tuberculosis Beijing genotype varies in prevalence in different regions of China and is solely associated with RMP resistance.