Study of Genetic Stability of Human Bone Marrow Multipotent Mesenchymal Stromal Cells
Study of Genetic Stability of Human Bone Marrow Multipotent Mesenchymal Stromal Cells
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DOI：
10.1007/s10517-011-1207-1
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发表时间：
2011-03-01
影响因子：
0.7
通讯作者：
Bochkov, N. P.
中科院分区：
文献类型：
--
作者：
Nikitina, V. A.;Osipova, E. Yu.;Bochkov, N. P.
对骨髓多能间充质干细胞的免疫表型、增殖率和遗传稳定性参数进行了研究。尽管第11-12代细胞增殖活性降低，但细胞保留了特征性免疫表型。评价了6、8、11号常染色体和性染色体的自发性非整倍体的发生率。两种培养的间充质基质细胞携带非整倍体细胞克隆进行检测：与染色体8三体和X染色体单体。结果表明，在体外培养条件下，骨髓间充质干细胞具有遗传转化和筛选染色体异常细胞的可能性。
Immunophenotype, proliferation rate, and genetic stability parameters of bone marrow multipotent mesenchymal stromal cells were studied. Despite the reduction of proliferative activity by passages 11-12, the cells retained the characteristic immunophenotype. The incidence of spontaneous aneuploidy for autosomes 6, 8, 11 and sex chromosomes was evaluated. Two cultures of mesenchymal stromal cells carrying aneuploid cell clones were detected: with chromosome 8 trisomy and X chromosome monosomy. The results indicate the possibility of genetic transformation and selection of mesenchymal stromal cells with abnormal karyotype during in vitro culturing.