Muc1, a mucin-like protein that is regulated by Mss10, is critical for pseudohyphal differentiation in yeast
Muc1, a mucin-like protein that is regulated by Mss10, is critical for pseudohyphal differentiation in yeast
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DOI：
10.1073/pnas.93.16.8419
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发表时间：
1996-08-06
影响因子：
11.1
通讯作者：
Pretorius, IS
中科院分区：
文献类型：
--
作者：
Lambrechts, MG;Bauer, FF;Pretorius, IS
酿酒酵母的假菌丝分化首先被描述为二倍体细胞对氮限制的反应。在这里，我们报告了单倍体和二倍体淀粉降解S。酿酒酵母菌株能够在充足的氮源存在下响应于碳限制而从酵母形式转变为丝状假菌丝形式。两个基因，MSS 10和MUC 1，被克隆，并显示参与假菌丝分化和侵入性生长。MSS 10的缺失导致假菌丝分化和侵入性生长的量大大减少，而MUC 1的缺失则完全消除了假菌丝分化和侵入性生长。Mss 10似乎是一个转录激活因子，响应营养限制和共调节MUC 1和STA 1 -3葡糖淀粉酶基因的表达，这是参与淀粉降解。MUC 1编码1367个氨基酸的蛋白质，含有几个富含丝氨酸/苏氨酸的重复序列。Mud是一种公认的整合膜结合蛋白，类似于哺乳动物粘蛋白样膜蛋白，其在癌细胞侵袭其他组织的能力中发挥作用。
Pseudohyphal differentiation in Saccharomyces cerevisiae was first described as a response of diploid cells to nitrogen limitation. Here we report that haploid and diploid starch-degrading S. cerevisiae strains were able to switch from a yeast form to a filamentous pseudohyphal form in response to carbon limitation in the presence of an ample supply of nitrogen. Two genes, MSS10 and MUC1, were cloned and shown to be involved in pseudohyphal differentiation and invasive growth. The deletion of MSS10 resulted in extremely reduced amounts of pseudohyphal differentiation and invasive growth, whereas the deletion of MUC1 abolished pseudohyphal differentiation and invasive growth completely. Mss10 appears to be a transcriptional activator that responds to nutrient limitation and coregulates the expression of MUC1 and the STA1-3 glucoamylase genes, which are involved in starch degradation. MUC1 encodes a 1367-amino acid protein, containing several serine/threonine-rich repeats. Mud is a putative integral membrane-bound protein, similar to mammalian mucin-like membrane proteins that have been implicated to play a role in the ability of cancer cells to invade other tissues.