Effect of a hyperthermic treatment on the pluripotent haemopoietic stem cell in normal and anaemic mice.
Effect of a hyperthermic treatment on the pluripotent haemopoietic stem cell in normal and anaemic mice.
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高温治疗对正常和贫血小鼠多能造血干细胞的影响。
DOI：
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发表时间：
1990
影响因子：
3.1
通讯作者：
A. Konings
中科院分区：
文献类型：
--
作者：
P. Wierenga;A. Konings
迄今为止，多能造血干细胞的高热敏感性尚不清楚，文献报道中已有的少数数据相互矛盾。这些研究的设置有两个主要缺点：(1)仅提供了CFU-S第9天的结果，而该试验是否能真实反映多能干细胞是值得怀疑的；(2)没有注意到播种效率的热影响，即将在脾脏中停留的干细胞的数量。本研究的重点是程序上的差异，并比较了干细胞的高温处理（60分钟，42℃）的结果，用CFU-S第9天和CFU-S第12天的方法，使用以下三种干细胞悬浮液，它们的增殖活性都不同：来自正常小鼠的骨髓和来自贫血小鼠的骨髓和脾脏细胞。此外，我们还研究了热处理前后的播种效率。静止的干细胞，用CFU-S第12天的方法检测，与活跃的循环干细胞相比，被证明对高温有抵抗力，而用CFU-S第9天的方法检测，干细胞在两种骨髓悬液中显示出相同的热敏性。在CFU-S第9天和第12天的实验中，活跃循环干细胞在热敏性方面没有显著差异，尽管骨髓和脾脏之间存在差异。热疗似乎影响脾脏CFU-S的播散效率；增加了1.73。（摘要删节250字）
Up to now, the hyperthermic sensitivity of pluripotent haemopoietic stem cells is unknown, and the few existing data from reports in the literature are conflicting. There are two main drawbacks in the set-up of those studies: (1) only CFU-S day 9 results were presented, whereas it is questionable if this assay gives a true reflection of the pluripotent stem cell, and (2) no attention has been paid to heat effects on the seeding efficiency, i.e. the amount of stem cells which will lodge in the spleen. The present study focused on the procedural differences and compared the results of a hyperthermic treatment (60 min, 42 degrees C) on the stem cells, assayed with the CFU-S day 9 and the CFU-S day 12 method, using the following three stem cell suspensions, all differing in their proliferative activity: bone marrow from normal mice and bone marrow and spleen cells from anaemic mice. Furthermore, we investigated the seeding efficiency before and after heat treatment. Resting stem cells, assayed with the CFU-S day 12 method, turned out to be resistant to hyperthermia as compared with the active cycling stem cells, while with the CFU-S day 9 assay the stem showed the same thermosensitivity in the two bone marrow suspensions. The active cycling stem cells do not significantly differ in thermosensitivity, in CFU-S day 9 and day 12 assays, although there is a difference between bone marrow and spleen. Hyperthermia appears to influence the seeding efficiency for spleen CFU-S; an increase of 1.73 was observed.(ABSTRACT TRUNCATED AT 250 WORDS)
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影响因子：
11.2
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影响因子：
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作者：
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通讯作者：
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