Drop-to-Drop Variation in the Cellular Components of Fingerprick Blood Implications for Point-of-Care Diagnostic Development
Drop-to-Drop Variation in the Cellular Components of Fingerprick Blood Implications for Point-of-Care Diagnostic Development
复制标题
DOI：
10.1309/ajcp1l7dkmpchpeh
复制
发表时间：
2015-12-01
影响因子：
3.5
通讯作者：
Richards-Kortum, Rebecca R.
中科院分区：
文献类型：
--
作者：
Bond, Meaghan M.;Richards-Kortum, Rebecca R.
目的：指点采血通常用于点血检测，但很少有研究评估连续点血获得的参数的变异性，这可能会给临床决策和评估点血的准确性带来问题。方法：我们使用血细胞分析仪分析11名献血者连续6滴点血中的血红蛋白浓度、总白细胞计数、三部分白细胞计数和血小板计数，结果：连续点血的平均百分比变异系数(CV)分别是静脉对照组的3.4倍、5.7倍、3倍、7.7倍和4倍。使用医疗点血色素测定仪测量的手指采血的平均百分比变异系数高达静脉血的5倍。当采血量等于或大于60~100时，血液参数随采血量的增加而波动在仪器变异性范围内。结论：这些数据提示在使用单滴指采血液测量时要谨慎。
Objectives: Blood obtained via fingerprick is commonly used in point-of-care assays, but few studies have assessed variability in parameters obtained from successive drops of fingerprick blood, which may cause problems for clinical decision making and for assessing accuracy of point-of-care tests.Methods: We used a hematology analyzer to analyze the hemoglobin concentration, total WBC count, three-part WBC differential, and platelet count in six successive drops of blood collected from one fingerprick from each of 11 donors, and we used a hemoglobinometer to measure the hemoglobin concentration of 10 drops of fingerprick blood from each of 7 donors.Results: The average percent coefficient of variation (CV) for successive drops of fingerprick blood was higher by up to 3.4 times for hemoglobin, 5.7 times for WBC count, 3 times for lymphocyte count, 7.7 times for granulocyte count, and 4 times for platelets than in venous controls measured using a hematology analyzer. The average percent CV for fingerprick blood was up to 5 times higher for hemoglobin than venous blood measured using a point-of-care hemoglobinometer. Fluctuations in blood parameters with increasing volume of fingerprick blood are within instrument variability for volumes equal to or greater than 60 to 100,mu L.Conclusions: These data suggest caution when using measurements from a single drop of fingerprick blood.