Protective immunity induced by a DNA vaccine encoding Eimeria tenella rhomboid against homologous challenge
Protective immunity induced by a DNA vaccine encoding Eimeria tenella rhomboid against homologous challenge
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编码菱形艾美耳球虫的 DNA 疫苗诱导的针对同源攻击的保护性免疫
DOI：
10.1007/s00436-012-3132-2
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发表时间：
2013-01-01
影响因子：
2
通讯作者：
Zhang, Xichen
中科院分区：
文献类型：
--
作者：
Liu, Yingli;Zheng, Jun;Zhang, Xichen
顶复门中的菱形蛋白与宿主细胞的入侵过程有关。为了评价该蛋白在诱导保护性免疫中的潜力，构建了编码柔嫩艾美耳球虫菱形体的DNA疫苗pVAX 1-Rho。重组蛋白在Hela细胞中得到表达，并通过间接免疫荧光和Western blotting分析进行验证。在体内试验中，将1周龄雏鸡随机分为3组。实验组用DNA疫苗免疫鸡，对照组用pVAX 1质粒单独免疫或用无菌水免疫鸡。加强剂量后两周，所有鸡口服接种5 × 10（4）个孢子化的E. tenella。研究了该疫苗的宿主免疫力和保护力。评估肉鸡中的柔嫩艾美耳球虫攻击。结果显示，pVAX 1-Rho组特异性抗体水平、白细胞介素2（IL-2）、干扰素γ（IFN-γ）水平以及CD 4+和CD 8 + T淋巴细胞百分比均显著升高。攻毒试验表明，pVAX 1-Rho疫苗能减少卵囊排泄，减轻盲肠病变，提高增重，卵囊减少率达75.8%。这些结果表明，pVAX 1-Rho能够诱导体液和细胞免疫应答，并产生抗E. tenella感染
Rhomboid protein in Apicomplexa was associated with the process of host cell invasion. To evaluate the potential of the protein in eliciting protective immunity against challenge, a DNA vaccine pVAX1-Rho encoding Eimeria tenella rhomboid was constructed. Recombinant protein was expressed in Hela cells and verified by indirect immunofluorescence and western blotting analysis. In vivo experiments, 1-week-old chickens were randomly divided into three groups. Experimental group of chickens were immunized with DNA vaccines while control group of chickens were injected with pVAX1 plasmid alone or sterile water. Two weeks following the booster dose, all chickens were inoculated orally with 5x10(4) sporulated oocysts of E. tenella. The host immunity and protective efficacy of this vaccine against E. tenella challenge in broilers were evaluated. Results showed that specific antibody, the levels of interleukin-2 (IL-2), interferon-gamma (IFN-gamma), and the percentages of CD4(+) and CD8(+) T lymphocyte cells were significantly increased in the pVAX1-Rho group. Challenge experiments demonstrated that pVAX1-Rho vaccination could reduce oocyst excretion, decrease cecal lesion, increase body-weight gains and provide chickens with oocysts decrease ratio around 75.8 %. These results suggest that the pVAX1-Rho was able to induce humoral and cellular responses and generate protective immunity against E. tenella infection.