Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism
Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism
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DOI：
10.1128/jvi.77.16.9084-9089.2003
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发表时间：
2003-08-01
影响因子：
5.4
通讯作者：
Britton, P
中科院分区：
文献类型：
--
作者：
Casais, R;Dove, B;Britton, P
使用我们的反向遗传学系统（R. Casais，V. Thiel，S. G. Siddell，D. Cavanagh，和 P. Britton，J. Virol. 75：12359-12369，2001）生成重组传染性支气管炎病毒（IBV）BeauR-M41（S），其中来自IBV M41-CK的刺突基因的胞外域区域被替换 IBV Beaudette 基因组的相应区域。 BeauR-M41(S) 在四种不同的细胞类型中获得了与 IBV M41-CK 相同的细胞向性表型，证明 IBV 刺突糖蛋白是细胞向性的决定因素。
A recombinant infectious bronchitis virus (IBV), BeauR-M41(S), was generated using our reverse genetics system (R. Casais, V. Thiel, S. G. Siddell, D. Cavanagh, and P. Britton, J. Virol. 75:12359-12369, 2001), in which the ectodomain region of the spike gene from IBV M41-CK replaced the corresponding region of the IBV Beaudette genome. BeauR-M41(S) acquired the same cell tropism phenotype as IBV M41-CK in four different cell types, demonstrating that the IBV spike glycoprotein is a determinant of cell tropism.