Radioimmunoassay for the middle region of human parathyroid hormone using an homologous antiserum with a carboxy-terminal fragment of bovine parathyroid hormone as radioligand.
Radioimmunoassay for the middle region of human parathyroid hormone using an homologous antiserum with a carboxy-terminal fragment of bovine parathyroid hormone as radioligand.
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使用以牛甲状旁腺激素羧基末端片段作为放射性配体的同源抗血清对人甲状旁腺激素中部区域进行放射免疫测定。
DOI：
10.1210/jcem-54-5-1017
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
J. Kirkland
中科院分区：
文献类型：
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作者：
L. Mallette;S. Tuma;R. Berger;J. Kirkland
本文报道了一种新型的人甲状旁腺激素（hPTH）放射免疫分析法。在山羊中产生抗hPTH的抗血清，并且放射性配体是牛PTH（bPTH）的羧基末端片段。虽然抗血清是二价的，但使用该片段作为示踪剂产生了对PTH分子中间区的单特异性测定。该测定可检测0.3fmol/管的完整hPTH-（1 -84）或0.1fmol合成hPTH-（44-68），但不检测hPTH-（1 -34）、hPTH-（53-84）或bPTH-（28-48）。在92名正常受试者中有85名可检测到免疫反应性PTH（iPTH），正常范围约为0.1-0.5 μU/ml hPTH研究标准75/549。22例连续严重甲状旁腺功能减退患者的iPTH检测不到，158例原发性甲状旁腺功能亢进患者中有147例的iPTH高于正常。在60例非甲状旁腺高钙血症患者中，iPTH平均比正常人低20%，但仍有80%可检测到。由于恶性肿瘤或其他非甲状旁腺原因引起的高钙血症的平均iPTH值难以区分。
A new type of RIA for human parathyroid hormone (hPTH) is described. The antiserum was generated in a goat against hPTH, and the radioligand is a carboxy-terminal fragment of bovine PTH (bPTH). Although the antiserum is bivalent, use of the fragment as tracer yields an assay monospe-cific for the midregion of the PTH molecule. The assay can detect 0.3 fmol/tube of intact hPTH-(l–84) or 0.1 fmol synthetic hPTH-(44–68), but does not detect hPTH-(l–34), hPTH-(53–84), or bPTH-(28–48). Immunoreactive PTH (iPTH) was detectable in 85 of 92 normal subjects, with a normal range of about 0.1–0.5 µU/ml hPTH Research Standard 75/549. iPTH was undetectable in 22 consecutive severely hypoparathyroid patients and was above normal in 147 of 158 patients with primary hyperparathyroidism. In 60 patients with nonparathy-roid hypercalcemia, iPTH averaged 20% lower than in normal subjects, but was still detectable in 80%. Mean iPTH values in hypercalcemia due to malignancy or other nonparathyroid causes were indistinguishable...