Identification of novel E2F1-regulated genes by microarray
Identification of novel E2F1-regulated genes by microarray
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DOI：
10.1006/abbi.2002.2761
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发表时间：
2002-03-15
影响因子：
3.9
通讯作者：
Cress, WD
中科院分区：
文献类型：
--
作者：
Ma, YH;Croxton, R;Cress, WD
E2F途径被提出用来调控参与从静止状态向DNA合成转变的基因。然而，这一假说还没有在基因组规模上得到严格的检验。为此，我们用表达E2F1的腺病毒感染静止的不表达E2F1的小鼠成纤维细胞，并使用高密度芯片检测了6000多个基因的表达。微阵列结果清楚地支持当前的范式；然而，他们表明E2F1也可能调节未预料到的细胞功能，包括参与细胞凋亡、信号转导、转录控制和膜生物学的途径。最令人惊讶的是，我们发现了一些受E2F1表达抑制的基因，这表明E2F1可能通过一种未知的机制抑制许多基因的转录。(C)2002年埃尔塞维尔科学公司(美国)。
The E2F pathway has been proposed to regulate genes involved in the transition from quiescence into DNA synthesis. However, this hypothesis has not been rigorously tested on a genomic scale. Toward this end, we have infected quiescent mouse fibroblasts, which do not express E2F1, with an E2F1-expressing adenovirus and examined the expression of more than 6000 genes using high-density microarrays. Microarray results clearly support the current paradigm; however, they suggest that E2F1 may also regulate unanticipated cellular functions including pathways involved in apoptosis, signal transduction, transcriptional control, and membrane biology. Most surprisingly, we identified a number of genes that are repressed by E2F1 expression, suggesting that E2F1 may have the potential to repress transcription of numerous genes through an unknown mechanism. (C) 2002 Elsevier Science (USA).