Comparison of 23S polymerase chain reaction-restriction fragment length polymorphism and amplified fragment length polymorphism techniques as typing systems for thermophilic campylobacters.
Comparison of 23S polymerase chain reaction-restriction fragment length polymorphism and amplified fragment length polymorphism techniques as typing systems for thermophilic campylobacters.
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23S 聚合酶链反应限制性片段长度多态性和扩增片段长度多态性技术作为嗜热弯曲杆菌分型系统的比较。
DOI：
10.1111/j.1574-6968.2002.tb11209.x
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发表时间：
2002
影响因子：
2.1
通讯作者：
J. Hernández
中科院分区：
文献类型：
--
作者：
Y. Moreno;M. Ferrús;Alicia Vanoostende;Manuel Hernández;R. Montes;J. Hernández
本研究采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)和扩增片段长度多态性(AFLP)分子分型技术相结合的方法，对临床和家禽样品中分离的嗜热弯曲杆菌进行了分子分型。对69株菌株的23S PCR-RFLP指纹图谱分析表明，该菌株具有较好的分型能力。通过带型的数值分析，在100%连锁水平上定义了11种不同的类型。在种水平上实现了空肠弯曲菌和结肠弯曲菌的分化，但这些图谱与菌株的来源之间没有明显的关系。对分离株的简化AFLP分析揭示了66种不同的带型。结果显示，所研究的菌株之间具有高度的多样性。所有菌株分为8个主要类型，它们之间的同源性为69%。亚种水平上的分化是可能的，但AFLP图谱与菌株的来源之间没有明显的关系。联合应用23S PCR-RFLP和单酶AFLP方法可用于确定嗜热弯曲杆菌的分类和流行病学关系。
In this study, we evaluated the combination of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and amplified fragment length polymorphism (AFLP) molecular typing techniques for the analysis of thermophilic campylobacter species isolated from clinical and poultry samples. 23S PCR-RFLP analysis performed to fingerprint 69 strains exhibited an excellent level of typability. Eleven different types were defined at 100% linkage level following numerical analysis of band patterns. Differentiation of Campylobacter jejuni and Campylobacter coli at species level was achieved although no significant relationship could be observed between the profiles and the origin of the strains. Simplified AFLP analysis of the isolates disclosed the presence of 66 different banding patterns. The resulting dendrogram showed a high diversity among the strains studied. All the isolates were grouped within eight main types with a 69% homology degree among them. Differentiation at subspecies level was possible but no significant relationship could be observed between the AFLP profiles and the origin of the strains. When used in combination, 23S PCR-RFLP and single-enzyme AFLP methods can be applied to determine taxonomic and epidemiological relationships among thermophilic campylobacters.