Imaging of a localized bacterial infection with endogenous thymidine kinase using radioisotope-labeled nucleosides
Imaging of a localized bacterial infection with endogenous thymidine kinase using radioisotope-labeled nucleosides
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DOI：
10.1016/j.ijmm.2011.11.002
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发表时间：
2012-03-01
影响因子：
4.1
通讯作者：
Kang, Joo Hyun
中科院分区：
文献类型：
--
作者：
Jang, Su Jin;Lee, Yong Jin;Kang, Joo Hyun
无创成像方法对细菌感染的重要性已被广泛认识。为了获得放射性同位素标记核苷的细菌感染成像，用[I-125]5-碘-1-（2'-氟-2'-脱氧- β -d -阿拉伯糖脲基）尿嘧啶（[I-125]FIAU）或3'-脱氧-3'[F-18]氟胸苷（[F-18]FLT）测定鼠伤寒沙门菌的细菌胸苷激酶（tk）活性。用[I-125]FIAU或[F-18]FLT分别对BALB/c小鼠感染模型进行小动物单光子发射计算机断层扫描（SPECT）或正电子发射断层扫描（PET）成像。[I-125]FIAU或[F-18]FLT在两种菌株中的累积放射性随培养时间或细菌数量的增加呈线性增加的趋势。图像清楚地显示在细菌感染部位[I-125]FIAU和[F-18]FLT的高摄取。[F-18]感染部位FLT摄取为7.286 +/- 2.405，未感染部位为0.519 +/- 0.561。根据生物分布数据测定，注射[I-125]FIAU后4 h，感染区与未感染区相对活性比为2.98。总之，通过[I-125]FIAU或[F-18]FLT的细胞摄取和成像证实了细菌的tk活性。因此，可以使用放射性同位素标记的核苷与核医学成像方式监测活体小鼠的局部细菌感染。(C) 2012 Elsevier GmbH版权所有。
The importance of noninvasive imaging methods to bacterial infections is widely recognized. To obtain bacterial infection imaging with radioisotope-labeled nucleosides, bacterial thymidine kinase (tk) activities of Salmonella typhimurium with [I-125]5-iodo-1-(2'-fluoro-2'-deoxy-beta-D-arabinofuranosyl)uracil ( [I-125]FIAU) or 3'-deoxy-3'[F-18]fluorothymidine ([F-18]FLT) were measured. The infection model in BALB/c mice was imaged with [I-125]FIAU or [F-18]FLT using small-animal Single Photon Emission Computed Tomography (SPECT) or Positron Emission Tomography (PET), respectively.The accumulated radioactivity of [I-125]FIAU or [F-18]FLT in the two strains showed a linearly increased pattern with increasing incubation time or bacterial numbers. The image clearly demonstrated a high uptake of [I-125]FIAU and [F-18]FLT in the bacterial infection site. [F-18]FLT uptake in the infection site of was 7.286 +/- 2.405, whereas that in the uninfected site was 0.519 +/- 0.561. The relative activity ratio of the infected region in relation to the uninfected region was 2.98 at 4 h after an injection with [I-125]FIAU determined by biodistribution data.In conclusion, the bacterial tk activity was confirmed by the cellular uptake and imaging with [I-125]FIAU or [F-18]FLT. Therefore, a localized bacterial infection in living mice can be monitored using radioisotope-labeled nucleosides with a nuclear medicine imaging modality. (C) 2012 Elsevier GmbH. All rights reserved.