A novel genotyping scheme for Vibrio parahaemolyticus with combined use of large variably-presented gene clusters (LVPCs) and variable-number tandem repeats (VNTRs)
A novel genotyping scheme for Vibrio parahaemolyticus with combined use of large variably-presented gene clusters (LVPCs) and variable-number tandem repeats (VNTRs)
复制标题
结合使用大型可变呈现基因簇 (LVPC) 和可变数量串联重复序列 (VNTR) 的副溶血性弧菌的新型基因分型方案。
DOI：
10.1016/j.ijfoodmicro.2011.06.014
复制
发表时间：
2011-09-15
影响因子：
5.4
通讯作者：
Zhou, Dongsheng
中科院分区：
文献类型：
--
作者：
Xiao, Xiao;Yan, Yanfeng;Zhou, Dongsheng
使用 PCR 和凝胶或毛细管电泳，在 251 个副溶血弧菌全局菌株中筛选了总共 18 个可变呈递基因簇 (LVPC) 和 9 个先前表征的可变数目串联重复序列 (VNTR) 以及所有已知毒力标记的频率和/或拷贝数。据此建立了结合使用 LVPC 和 VNTR 的两步基因分型方法。 LVPC 和毒力标记的频率分布主要用于将菌株分为六种具有不同潜在致病性的不同复合体。使用 VNTR 进一步分析来自每个复合物的菌株，以对菌株进行更详细的区分。用这种两阶段方法建立的大量菌株的遗传指纹样数据库对于副溶血性弧菌的鉴定、基因分型、起源追踪和风险评估非常有用。 (C) 2011 Elsevier B.V. 保留所有权利。
A total of 18 variably-presented gene clusters (LVPCs) and nine previously characterized variable-number tandem repeats (VNTRs), and all known virulence markers were screened for their frequency and/or copy number in 251 global strains of Vibrio parahaemolyticus using PCR and gel or capillary electrophoresis. A two-step genotyping approach combining the use of LVPCs and VNTRs was established accordingly. The frequency profiles of LVPCs and virulence markers were primarily used to group the strains into six distinct complexes with different potential pathogenicity natures. The strains from each of these complexes were further analyzed with VNTRs to give a much more detailed discrimination of the strains. A genetic fingerprint-like database of a large collection of strains established with this two-stage approach would be very useful for identification, genotyping, origin tracing, and risk estimation of V. parahaemolyticus. (C) 2011 Elsevier B.V. All rights reserved.