Physiological and biochemical response of Aedes aegypti tolerance to Bacillus thuringiensis
Physiological and biochemical response of Aedes aegypti tolerance to Bacillus thuringiensis
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埃及伊蚊对苏云金芽孢杆菌耐受的生理生化反应
DOI：
10.1080/09583157.2015.1089216
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发表时间：
2016
影响因子：
1.4
通讯作者：
Guan Xiong
中科院分区：
文献类型：
--
作者：
Wu Songqing;Wu Wei;Zhu Xiaoli;Liu Zhaoxia;Rebeca Carballar-Lejarazu;Fu Tao;Han Chaoqiang;Shao Ensi;Pan Xiaohong;Huang Zhipeng;Xu Lei;Zou Shuangquan;Zhang Lingling;Guan Xiong
芽孢杆菌thuringiensissubsp。以色列人（Bti）是唯一一种生态友好的生物可降解杀虫剂，用于控制蚊媒疾病。我们的研究目的是确定蚊子解毒酶是否在登革热媒介埃及伊蚊的Bti耐受机制中发挥重要作用。分析了几种酶，如淀粉酶、细胞色素P450、Na+/K+- atp酶、乙酰胆碱酯酶、蛋白酶和谷胱甘肽转移酶（GST），并测定了活性水平。Bti处理后的埃及伊蚊幼虫。Bti暴露显著提高了淀粉酶（183.2%）、细胞色素P450（177.5%）和Na+/K+- atp酶（142.9%）的活性。另一方面，乙酰胆碱酯酶和GST活性分别下降了8.6%和11.4%，对蛋白酶总活性水平无显著影响。我们认为，淀粉酶、细胞色素P450、Na+/K+- atp酶、乙酰胆碱酯酶、蛋白酶和GST活性的变化可能与Bti杀虫机制有关。本研究为Bti耐受性解毒酶的研究提供了依据。
Bacillus thuringiensissubsp.israelensis(Bti) represents the only eco-friendly bio-degradable insecticide for mosquito-borne disease control. Our research aims to identify if mosquito detoxification enzymes play an important role in Bti tolerance mechanisms in the dengue vector,Aedes aegypti. Several enzymes, such as amylase, cytochromes P450, Na+/K+-ATPase, acetylcholinesterase, protease and glutathioneS-transferase (GST) were analysed and level of activity determined inAe. aegyptilarvae after Bti treatment. Bti exposure significantly increased the level of amylase (183.2%) as well as the activity of cytochromes P450 (177.5%), and Na+/K+-ATPase (142.9%). On the other hand, there was a decrease of 8.6% and 11.4% in acetylcholinesterase and GST activity, and no significant effect in the total level of protease activity. We suggest that the variation in amylase, cytochromes P450, Na+/K+-ATPase, acetylcholinesterase, protease and GST activity may be associated with the Bti insecticidal mechanism. This study provides the basis of detoxifying enzymes in Bti tolerance.