Blood cell characterization and transcriptome analysis reveal distinct immune response and host resistance of different ploidy cyprinid fish following Aeromonas hydrophila infection
Blood cell characterization and transcriptome analysis reveal distinct immune response and host resistance of different ploidy cyprinid fish following Aeromonas hydrophila infection
复制标题
血细胞特征和转录组分析揭示了不同倍性鲤鱼在嗜水气单胞菌感染后的独特免疫反应和宿主抵抗力
DOI：
10.1016/j.fsi.2021.12.024
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发表时间：
2022
影响因子：
4.7
通讯作者：
Qing-Feng Liu
中科院分区：
文献类型：
--
作者：
Ning-Xia Xiong;Jie Ou;Lan-Fen Fan;Xu-Ying Kuang;Zi-Xuan Fang;Sheng-Wei Luo;Zhuang-Wen Mao;Shao-Jun Liu;Shi Wang;Ming Wen;Kai-Kun Luo;Fang-Zhou Hu;Chang Wu;Qing-Feng Liu
Aeromonas hydrophila can pose a great threat to survival of freshwater fish. In this study,A. hydrophilainfection could decrease blood cell numbers, promote blood cell damage as well as alter the levels of alkaline phosphatase (ALP), lysozyme (LZM), aspartate aminotransferase (AST), total antioxidant capacity (T-AOC), total superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) in immune-related tissues of red crucian carp (RCC, 2 N = 100) and triploid cyprinid fish (3 N fish, 3 N = 150). In addition, the significant alternation of antioxidant status was observed in PBMCs isolated from RCC and 3 N following LPS stimulation. The core differential expression genes (DEGs) involved in apoptosis, immunity, inflammation and cellular signals were co-expressed differentially in RCC and 3 N followingA. hydrophilachallenge. NOD-like receptor (NLR) signals appeared to play a critical role inA. hydrophila-infected fish. DEGs of NLR signals in RCCah vs RCCctl were enriched in caspase-1-dependent Interleukin-1β (IL-1β) secretion, interferon (IFN) signals as well as cytokine activation, while DEGs of NLR signals in 3Nah vs 3Nctl were enriched in caspase-1-dependent IL-1β secretion and antibacterial autophagy. These results highlighted the differential signal regulation of different ploidy cyprinid fish to cope with bacterial infection.
