Quantitative cytomorphological comparison of SurePath and ThinPrep liquid-based cytology using high-grade urothelial carcinoma cells
Quantitative cytomorphological comparison of SurePath and ThinPrep liquid-based cytology using high-grade urothelial carcinoma cells
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DOI：
10.1111/cyt.12998
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发表时间：
2021-06-10
期刊：
影响因子：
1.3
通讯作者：
Ohsaki, Hiroyuki
中科院分区：
文献类型：
--
作者：
Okuda, Chihiro;Kyotake, Aiko;Ohsaki, Hiroyuki
目的在PARIS尿细胞学报告系统中，诊断高级别尿路上皮癌(HGUC)的重要细胞形态特征是核浆比(N：C)大于0.7，染色质增多，染色质粗大，核边界不规则。然而，使用SurePath玻片对HGUC细胞进行定量细胞形态学评估的情况很少见。因此，我们使用数字图像分析系统对SurePath玻片上的HGUC细胞进行了定量评估，并将这些数据与ThinPrep数据进行了比较。方法将相同的尿样分成两等份，用于制备SurePath和ThinPrep玻片。结果SurePath和ThinPrep玻片上分析的HGUC细胞总数为981个，ThinPrep玻片上分析的HGUC细胞总数为889个。SurePath上的染色质增厚和不规则的核边界明显比ThinPrep幻灯片上的严重。相反，N：C比率在不同方法之间没有不同。结论在SurePath和ThinPrep玻片上均以N：C比值作为TPS的主要标准是合理的，N：C比值临界值为0.7是适合于鉴别HGUC细胞的。然而，不同的处理方法，染色质增厚和不规则的核边界的严重程度不同。
Objective In The Paris System for Reporting Urinary Cytology (TPS), the important cytomorphological features for diagnosing high-grade urothelial carcinoma (HGUC) are a nuclear-to-cytoplasmic (N:C) ratio exceeding 0.7, hyperchromasia, coarse chromatin, and irregular nuclear borders. However, quantitative cytomorphological assessments of HGUC cells using SurePath slides are rare. Therefore, we evaluated HGUC cells on SurePath slides quantitatively using a digital image analysis system and compared these data with ThinPrep data.Methods The same urine samples were divided into two aliquots and used to prepare SurePath and ThinPrep slides. We used ImageJ to measure the N:C ratio, hyperchromasia, and irregular nuclear borders for HGUC cells on SurePath and ThinPrep slides.Results The total number of analysed HGUC cells on SurePath slides was 981, versus 889 on ThinPrep slides. Hyperchromasia and irregular nuclear borders were significantly more severe on SurePath than on ThinPrep slides. Conversely, the N:C ratio did not differ between the methods. Additionally, HGUC cells with N:C ratios exceeding 0.7 were present on almost all slides for both methods.Conclusions Our data indicated the reasonableness of using the N:C ratio as the major criterion for TPS on both SurePath and ThinPrep slides, and an N:C ratio cut-off of 0.7 as suitable for identifying HGUC cells. However, the severity of hyperchromasia and irregular nuclear borders differed between the processing methods.