Cytoplasmic HYL1 modulates miRNA-mediated translational repression
Cytoplasmic HYL1 modulates miRNA-mediated translational repression
复制标题
细胞质 HYL1 调节 miRNA 介导的翻译抑制
DOI：
10.1093/plcell/koab090
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发表时间：
2021
期刊：
影响因子：
11.6
通讯作者：
Feijie Wu
中科院分区：
文献类型：
--
作者：
Xi Yang;Weiguo Dong;Wenqing Ren;Yuke He;Qiuxia Zhao;Feijie Wu
微小RNA（microRNAs，miRNAs）通过抑制靶mRNA来调控多种生物学过程。在植物中，miRNA通过mRNA切割和翻译抑制介导靶基因抑制。然而，这种翻译抑制背后的机制知之甚少。在这里，我们发现，拟南芥hyponastic LEAVES1（HYL1），一个核心组成部分的miRNA加工机制，调节miRNA介导的mRNA翻译，但不是miRNA的生物合成时，它定位在细胞质中。细胞质HYL 1定位于内质网并与Argonaute 1（AGO 1）和ALTERED MERISTEM PROGRAM1相关。在细胞质中，HYL 1监测AGO 1在多聚核糖体上的分布，与靶基因的mRNA结合，抑制它们的翻译，并部分挽救hyl1无效突变体的表型。这项研究揭示了HYL 1的另一个功能，并为植物基因调控机制提供了新的见解。
MicroRNAs (miRNAs) control various biological processes by repressing target mRNAs. In plants, miRNAs mediate target gene repression via both mRNA cleavage and translational repression. However, the mechanism underlying this translational repression is poorly understood. Here, we found thatArabidopsis thalianaHYPONASTIC LEAVES1 (HYL1), a core component of the miRNA processing machinery, regulates miRNA-mediated mRNA translation but not miRNA biogenesis when it localized in the cytoplasm. Cytoplasmic HYL1 localizes to the endoplasmic reticulum and associates with ARGONAUTE1 (AGO1) and ALTERED MERISTEM PROGRAM1. In the cytoplasm, HYL1 monitors the distribution of AGO1 onto polysomes, binds to the mRNAs of target genes, represses their translation, and partially rescues the phenotype of thehyl1null mutant. This study uncovered another function of HYL1 and provides insight into the mechanism of plant gene regulation.